Research Centre Information Centre AgeFactDB
Release 1 - Aug 15, 2013

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Ageing Phenotype Observations List—Data Type 2

NOTE:

We distinguish between different types of ageing relevance evidence. Experimental studies on ageing factors result in observations with experimental ageing relevance evidence (1). According to our definition of ageing factors a comparison of two situations, with and without the action of the ageing factor, is required. Almost all data in AgeFactDB is of this type. There are however also a few lifespan data for populations or species included that are certainly relevant to ageing with a slightly different meaning of the term ageing relevance, however. But in this case there is no intervention study and therefore these data cannot be assigned to an ageing factor (2). Furthermore, there are experimental studies where no significant effect of an assumed ageing factor was found (3). And finally there are genes included that are homologous to known ageing-relevant genes for which there is no experimental evidence. They are considered as putative ageing-relevant genes with computational evidence (4).
Order of importance:

  1. yes (Experimental Analysis)
  2. yes, but no ageing factor assigned (Experimental Analysis)
  3. no (Experimental Analysis)
  4. putative (Computational Analysis)
The color indicating the ageing relevance type of an observation may differ from the corresponding color for the ageing factor related to this observation. The reason is that for ageing factors associated with more than one observation, the most important observation color is chosen as ageing factor color. This is always the color with the lowest number in the order given above.

Ageing phenotype observations are considered as experimental analysis. They confirm or reject the ageing relevance for particular ageing factor(s). For genes that are homologous to known ageing-relevant genes there is thus far no experimental evidence. They are considered as candidate genes.
Due to the different data structures of ageing phenotype observation data integrated from other databases we subdivide these observations into type 1 and 2. Observations of type 1 contain the phenotype data mostly unseparated within a single description. Observations of type 2 contain lifespan data in a more structured form (e.g.: separated into lifespan effect, lifespan change, lifespan value). Currently there might be an overlap between the observations of type 1 and 2 from the same source, namely GenAge. But we are working on a reduction of this overlap.

Ageing Relevance:
101 ... 200 [previous icon] [next icon]
# Observation Stable ID Species Strain Gene Compound Other Ageing Factor Significant Lifespan Effect Change Observed Lifespan Reference Lifespan Lifespan Unit Measure Temperature Temperature Unit Sex/Mating Type Description Author Year PubMed DOI Source
101 OB_001221 Caenorhabditis elegans N2
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
daf-2Caenorhabditis elegans175410loss of function-unknownunknown
gpa-11Caenorhabditis elegans173759loss of function-unknownunknown
odr-3Caenorhabditis elegans179806loss of function-unknownunknown
increased 170.00% 48.6 18 days mean hermaphrodite Loss of function mutations increased lifespan 170%. Lans et al. 2007 17187771 [DOI icon]doi:10.1016/j.ydbio.2006.11.028 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 2544)
102 OB_006129 Caenorhabditis elegans
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
age-1Caenorhabditis elegans174762RNA interference -wildtypeyes
increased 170.00% Average 170% mean lifespan extension Hamilton et al. 2005 15998808 [DOI icon]doi:10.1101/gad.1308205 [GenAge icon]GenAge
103 OB_002249 Caenorhabditis elegans N2
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
atp-3Caenorhabditis elegans172195RNAi knockdown-unknownunknown
daf-2Caenorhabditis elegans175410loss of function(e1370)IIIunknownunknown
increased 168.42% 40.8 15.2 days mean 25 hermaphrodite For calculating relative effect of lifespan in mutant background, an average of all N2 data in Supplemental Table I was used since no experiment matched control was provided. RNA knockdown increased lifespan of daf-2 mutants by 63% relative to daf-2 mutants alone. Hansen et al. 2005 16103914 [DOI icon]doi:10.1371/journal.pgen.0010017 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 3524)
104 OB_002300 Caenorhabditis elegans N2
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
daf-2Caenorhabditis elegans175410loss of functione1370unknownunknown
increased 166.67% 56 21 days max 25 hermaphrodite Mutation leads to increased (166%) maximum lifespan. Feng et al. 2001 11709184 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 3574)
105 OB_000021 Caenorhabditis elegans N2
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
daf-2Caenorhabditis elegans175410RNAi knockdown-unknownunknown
vhl-1Caenorhabditis elegans181321deletion / nullok161unknownunknown
increased 165.82% 52.1 19.6 days mean 20 hermaphrodite RNAi knock-down of daf-2 increased life span additively with deletion of vhl-1. These data support the model that VHL-1 and insulin-like signaling act in parallel pathways to modulate longevity. Mehta et al. 2009 19372390 [DOI icon]doi:10.1126/science.1173507 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 1019)
106 OB_000916 Caenorhabditis elegans N2
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
daf-12Caenorhabditis elegans181263unknownm20unknownunknown
daf-2Caenorhabditis elegans175410unknowne979unknownunknown
increased 165.64% 43.3 16.3 days median 22.5 hermaphrodite 15 temperature-sensitive and 1 nonconditional daf-2 allele were characterized to investigate the extent of daf-2 mutant defects and to examine whether specific mutant traits correlate with each other. The greatest longevity seen in daf-2 mutant adults was approximately three times that of wild type. The temperature-sensitive daf-2 mutants fell into two overlapping classes, including eight class 1 mutants, which are Daf-c, Age, and Itt, and exhibit low levels of L1 arrest at 25.5 degrees. Seven class 2 mutants also exhibit the class 1 defects as well as some or all of the following: reduced adult motility, abnormal adult body and gonad morphology, high levels of embryonic and L1 arrest, production of progeny late in life, and reduced brood size. The strengths of the Daf-c, Age, and Itt phenotypes largely correlated with each other but not with the strength of class 2-specific defects. This suggests that the DAF-2 receptor is bifunctional. Gems et al. 1998 9725835 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 2223)
107 OB_002301 Caenorhabditis elegans N2
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
daf-2Caenorhabditis elegans175410loss of functione1370unknownunknown
increased 165.32% 32.9 12.4 days mean 25 hermaphrodite Mutation leads to increased (166%) mean lifespan. Feng et al. 2001 11709184 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 3575)
108 OB_000859 Caenorhabditis elegans N2
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
osm-3Caenorhabditis elegans177141unknownp802unknownunknown
Other Ageing Factor NameAdditional Information
Dietary restrictionBacterial deprivation beginning at the 4th day of adulthood.
increased 165.22% 61 23 days median 20 hermaphrodite Dietary restriction by bacterial deprivation significantly increased the life span of long-lived mutants defective for chemosensary function. Smith et al. 2008 18457595 [DOI icon]doi:10.1186/1471-213X-8-49 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 2140)
109 OB_000860 Caenorhabditis elegans N2
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
tax-4Caenorhabditis elegans176297unknownp678unknownunknown
Other Ageing Factor NameAdditional Information
Dietary restrictionBacterial deprivation beginning at the 4th day of adulthood.
increased 165.22% 61 23 days median 20 hermaphrodite Dietary restriction by bacterial deprivation significantly increased the life span of long-lived mutants defective for chemosensary function. Smith et al. 2008 18457595 [DOI icon]doi:10.1186/1471-213X-8-49 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 2141)
110 OB_001684 Caenorhabditis elegans N2
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
daf-2Caenorhabditis elegans175410unknowne1391unknownunknown
increased 164.17% 63.4 24 days median 15 hermaphrodite 15 temperature-sensitive and 1 nonconditional daf-2 allele were characterized to investigate the extent of daf-2 mutant defects and to examine whether specific mutant traits correlate with each other. The greatest longevity seen in daf-2 mutant adults was approximately three times that of wild type. The temperature-sensitive daf-2 mutants fell into two overlapping classes, including eight class 1 mutants, which are Daf-c, Age, and Itt, and exhibit low levels of L1 arrest at 25.5 degrees. Seven class 2 mutants also exhibit the class 1 defects as well as some or all of the following: reduced adult motility, abnormal adult body and gonad morphology, high levels of embryonic and L1 arrest, production of progeny late in life, and reduced brood size. The strengths of the Daf-c, Age, and Itt phenotypes largely correlated with each other but not with the strength of class 2-specific defects. This suggests that the DAF-2 receptor is bifunctional. Gems et al. 1998 9725835 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 3015)
111 OB_002346 Caenorhabditis elegans N2
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
daf-2Caenorhabditis elegans175410loss of functione1370unknownunknown
kri-1Caenorhabditis elegans191275RNAi knockdown-unknownunknown
increased 163.74% 48 18.2 days mean 20 hermaphrodite RNA interference had no significant effect relative to mutant lifespan. Berman et al. 2006 16530050 [DOI icon]doi:10.1016/j.cell.2006.01.039 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 3618)
112 OB_002145 Caenorhabditis elegans N2
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
daf-2Caenorhabditis elegans175410loss of function(e1370)IIIunknownunknown
rha-2Caenorhabditis elegans176207RNAi knockdown-unknownunknown
increased 163.54% 50.6 19.2 days mean 20 hermaphrodite For calculating relative effect of lifespan in mutant background, an average of all N2 data in Supplemental Table I was used since no experiment matched control was provided. RNA knockdown increased daf-2 lifespan by 10% relative to daf-2 lifespan alone. Hansen et al. 2005 16103914 [DOI icon]doi:10.1371/journal.pgen.0010017 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 3430)
113 OB_001220 Caenorhabditis elegans N2
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
daf-2Caenorhabditis elegans175410loss of function-unknownunknown
gpa-11Caenorhabditis elegans173759loss of function-unknownunknown
increased 162.78% 47.3 18 days mean hermaphrodite Loss of function mutations increased lifespan 163%. Lans et al. 2007 17187771 [DOI icon]doi:10.1016/j.ydbio.2006.11.028 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 2543)
114 OB_002141 Caenorhabditis elegans N2
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
daf-2Caenorhabditis elegans175410loss of function(e1370)IIIunknownunknown
pat-6Caenorhabditis elegans176848RNAi knockdown-unknownunknown
increased 161.98% 50.3 19.2 days mean 20 hermaphrodite For calculating relative effect of lifespan in mutant background, an average of all N2 data in Supplemental Table I was used since no experiment matched control was provided. RNA knockdown increased daf-2 lifespan by 22% relative to daf-2 lifespan alone. Hansen et al. 2005 16103914 [DOI icon]doi:10.1371/journal.pgen.0010017 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 3427)
115 OB_001100 Caenorhabditis elegans N2
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
daf-2Caenorhabditis elegans175410deletion / nulle1370unknownunknown
increased 161.70% 49.2 18.8 days mean 20 hermaphrodite Several (but not all) mutations that cause defects in sensory cilia or their support cells, or in sensory signal transduction, extend lifespan. These mutations do not further extend long-lived daf-2 mutants Apfeld et al. 1999 10617200 [DOI icon]doi:10.1038/45544 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 2419)
116 OB_000854 Caenorhabditis elegans N2
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
che-11Caenorhabditis elegans179666unknowne1810unknownunknown
Other Ageing Factor NameAdditional Information
Dietary restrictionBacterial deprivation beginning at the 4th day of adulthood.
increased 160.87% 60 23 days median 20 hermaphrodite Dietary restriction by bacterial deprivation significantly increased the life span of long-lived mutants defective for chemosensary function. Smith et al. 2008 18457595 [DOI icon]doi:10.1186/1471-213X-8-49 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 2136)
117 OB_000867 Saccharomyces cerevisiae PSY316AR
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
SIR2Saccharomyces cerevisiae851520deletion / null-unknownunknown
Other Ageing Factor NameAdditional Information
Dietary restrictionReduced glucose in the medium to 0.05%
increased 160.00% 13 5 days median 30 matalpha Deletion of sir2, hst1, hst2, hst3, hst4 or all 5 sirtuins at the same time had no effect on chronological life span and did not reduce life span extension from dietary restriction at 0.5% or 0.05% glucose. Murakami et al. 2008 18314444 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 2172)
118 OB_000868 Saccharomyces cerevisiae PSY316AR
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
SIR2Saccharomyces cerevisiae851520deletion / null-unknownunknown
Other Ageing Factor NameAdditional Information
Dietary restrictionReduced glucose in the medium to 0.5%
increased 160.00% 13 5 days median 30 matalpha Deletion of sir2, hst1, hst2, hst3, hst4 or all 5 sirtuins at the same time had no effect on chronological life span and did not reduce life span extension from dietary restriction at 0.5% or 0.05% glucose. Murakami et al. 2008 18314444 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 2173)
119 OB_004819 Saccharomyces cerevisiae PSY316AR
Other Ageing Factor NameAdditional Information
Dietary restrictionReduced glucose in the medium to 0.05%
increased 160.00% 13 5 days median 30 matalpha Dietary restriction by reducing the glucose concentration of the medium from 2% to 0.5% or 0.05% increased chronological life span in the BY4742, PSY316AR, and W303AR5 strain backgrounds. Murakami et al. 2008 18314444 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 2164)
120 OB_004820 Saccharomyces cerevisiae PSY316AR
Other Ageing Factor NameAdditional Information
Dietary restrictionReduced glucose in the medium to 0.5%
increased 160.00% 13 5 days median 30 matalpha Dietary restriction by reducing the glucose concentration of the medium from 2% to 0.5% or 0.05% increased chronological life span in the BY4742, PSY316AR, and W303AR5 strain backgrounds. Murakami et al. 2008 18314444 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 2165)
121 OB_002242 Caenorhabditis elegans N2
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
daf-2Caenorhabditis elegans175410loss of function(e1370)IIIunknownunknown
rab-10Caenorhabditis elegans266836RNAi knockdown-unknownunknown
increased 158.55% 39.3 15.2 days mean 25 hermaphrodite For calculating relative effect of lifespan in mutant background, an average of all N2 data in Supplemental Table I was used since no experiment matched control was provided. RNA knockdown increased lifespan of daf-2 mutants by 46% relative to daf-2 mutants alone. Hansen et al. 2005 16103914 [DOI icon]doi:10.1371/journal.pgen.0010017 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 3518)
122 OB_002416 Caenorhabditis elegans N2
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
daf-2Caenorhabditis elegans175410RNAi knockdown-unknownunknown
lin-14Caenorhabditis elegans181337gain of functionn179tsunknownunknown
increased 158.43% 23 8.9 days mean 25 hermaphrodite RNA knockdown increased lifespan relative to both wild type and mutant lifespan. Boehm et al. 2005 16373574 [DOI icon]doi:10.1126/science.1115596 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 3681)
123 OB_004916 Saccharomyces cerevisiae YPK9
Other Ageing Factor NameAdditional Information
Dietary restrictionMedia glucose reduced from 2% to 0.1%.
Dietary restrictionNonessential amino acids removed from media.
increased 157.98% 30.7 11.9 divisions mean 30 mata Combining two forms of dietary restriction, glucose restriction and nonessential amino acid elimination, increases life span by 158.0% Jiang et al. 2002 12213553 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 4462)
124 OB_001026 Caenorhabditis elegans N2
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
daf-2Caenorhabditis elegans175410loss of functione1370unknownunknown
sir-2.1Caenorhabditis elegans177924over-expressiongeEx3unknownunknown
increased 157.92% 47.2 18.3 days mean 20 hermaphrodite Transgenic overexpression of sir-2.1 from an extrachromosomal or integrated multicopy array extends life span. Other phenotypes: Life span extension is suppressed by a mutation in daf-16 and does not synergize with daf-2. Overexpression of sir-2.1 synergizes with the TGF-beta mutations daf-4 and daf-1 for dauer formation. Tissenbaum et al. 2001 11242085 [DOI icon]doi:10.1038/35065638 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 2347)
125 OB_002347 Caenorhabditis elegans N2
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
daf-2Caenorhabditis elegans175410loss of functione1370unknownunknown
rrf-3Caenorhabditis elegans174315loss of functionpk1426unknownunknown
increased 157.69% 46.9 18.2 days mean 20 hermaphrodite Double mutation extended lifespan approximately 158%. Berman et al. 2006 16530050 [DOI icon]doi:10.1016/j.cell.2006.01.039 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 3619)
126 OB_002247 Caenorhabditis elegans N2
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
cchl-1Caenorhabditis elegans174713RNAi knockdown-unknownunknown
daf-2Caenorhabditis elegans175410loss of function(e1370)IIIunknownunknown
increased 157.24% 39.1 15.2 days mean 25 hermaphrodite For calculating relative effect of lifespan in mutant background, an average of all N2 data in Supplemental Table I was used since no experiment matched control was provided. RNA knockdown increased lifespan of daf-2 mutants by 45% relative to daf-2 mutants alone. Hansen et al. 2005 16103914 [DOI icon]doi:10.1371/journal.pgen.0010017 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 3522)
127 OB_003448 Caenorhabditis elegans N2
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
daf-2Caenorhabditis elegans175410loss of functione1370unknownunknown
increased 156.00% 64 25 days max 20 Maximum lifespan of daf-2 mutant was approximately 156% longer than control. Huang et al. 2011 21471153 [DOI icon]doi:10.1242/dev.057109 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 4874)
128 OB_003422 Caenorhabditis elegans N2
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
cdc-25.1Caenorhabditis elegans172353RNAi knockdown-unknownunknown
daf-2Caenorhabditis elegans175410loss of functione1371unknownunknown
Other Ageing Factor NameAdditional Information
Pathogen resistanceWorms were infected at 25 C by feeding on PA14 lawns.
increased 153.84% 343.7 135.4 mean 25 Lifespan of daf-2(e1371) mutant was approximately 154% longer than N2 control on cdc-25.1 RNAi background. Evans et al. 2008 18782349 [DOI icon]doi:10.1111/j.1474-9726.2008.00435.x [Lifespan Observations Database icon]Lifespan Observations Database (ID: 4847)
129 OB_002135 Caenorhabditis elegans N2
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
daf-2Caenorhabditis elegans175410RNAi knockdown-unknownunknown
daf-2Caenorhabditis elegans175410loss of function(e1370)IIIunknownunknown
increased 153.65% 48.7 19.2 days mean 20 hermaphrodite For calculating relative effect of lifespan in mutant background, an average of all N2 data in Supplemental Table I was used since no experiment matched control was provided. RNA knockdown had no significant effect on lifespan of daf-2 mutants. Hansen et al. 2005 16103914 [DOI icon]doi:10.1371/journal.pgen.0010017 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 3421)
130 OB_004908 Caenorhabditis elegans N2
Other Ageing Factor NameAdditional Information
Axenic mediumCulture on non-bacterial, semi-dened, axenic food sources.
increased 152.78% 36.4 14.4 days mean 24 hermaphrodite Comparisons of longevity of wild type and mutants grown on bacteria vs. axenic culture. Houthoofd et al. 2003 12954481 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 4331)
131 OB_002150 Caenorhabditis elegans N2
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
atp-4Caenorhabditis elegans179025RNAi knockdown-unknownunknown
daf-2Caenorhabditis elegans175410loss of function(e1370)IIIunknownunknown
increased 151.56% 48.3 19.2 days mean 20 hermaphrodite For calculating relative effect of lifespan in mutant background, an average of all N2 data in Supplemental Table I was used since no experiment matched control was provided. RNA knockdown increased daf-2 lifespan by 17% relative to daf-2 lifespan alone. Hansen et al. 2005 16103914 [DOI icon]doi:10.1371/journal.pgen.0010017 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 3435)
132 OB_001703 Caenorhabditis elegans N2
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
daf-2Caenorhabditis elegans175410unknownsa223unknownunknown
increased 151.53% 41 16.3 days median 22.5 hermaphrodite 15 temperature-sensitive and 1 nonconditional daf-2 allele were characterized to investigate the extent of daf-2 mutant defects and to examine whether specific mutant traits correlate with each other. The greatest longevity seen in daf-2 mutant adults was approximately three times that of wild type. The temperature-sensitive daf-2 mutants fell into two overlapping classes, including eight class 1 mutants, which are Daf-c, Age, and Itt, and exhibit low levels of L1 arrest at 25.5 degrees. Seven class 2 mutants also exhibit the class 1 defects as well as some or all of the following: reduced adult motility, abnormal adult body and gonad morphology, high levels of embryonic and L1 arrest, production of progeny late in life, and reduced brood size. The strengths of the Daf-c, Age, and Itt phenotypes largely correlated with each other but not with the strength of class 2-specific defects. This suggests that the DAF-2 receptor is bifunctional. Gems et al. 1998 9725835 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 3032)
133 OB_008072 Caenorhabditis elegans N2
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
daf-2Caenorhabditis elegans175410RNAi knockdown-wildtypeyes
increased 151.00% 70.3 28 day maximum hermaphrodite Zarse et al. 2012 22482728 [DOI icon]doi:10.1016/j.cmet.2012.02.013 [AgeFactDB Curator icon]AgeFactDB Curator
134 OB_001112 Caenorhabditis elegans N2
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
tax-4Caenorhabditis elegans176297unknownp678unknownunknown
Other Ageing Factor NameAdditional Information
Germ cell ablationZ2, Z3 laser ablation at L1 larval stage
increased 150.54% 46.1 18.4 days mean 20 hermaphrodite Several (but not all) mutations that cause defects in sensory cilia or their support cells, or in sensory signal transduction, extend lifespan. These mutations do not further extend long-lived daf-2 mutants Apfeld et al. 1999 10617200 [DOI icon]doi:10.1038/45544 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 2432)
135 OB_002140 Caenorhabditis elegans N2
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
daf-2Caenorhabditis elegans175410loss of function(e1370)IIIunknownunknown
pat-4Caenorhabditis elegans175175RNAi knockdown-unknownunknown
increased 150.52% 48.1 19.2 days mean 20 hermaphrodite For calculating relative effect of lifespan in mutant background, an average of all N2 data in Supplemental Table I was used since no experiment matched control was provided. RNA knockdown increased daf-2 lifespan by 16% relative to daf-2 lifespan alone. Hansen et al. 2005 16103914 [DOI icon]doi:10.1371/journal.pgen.0010017 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 3426)
136 OB_000020 Caenorhabditis elegans N2
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
daf-2Caenorhabditis elegans175410RNAi knockdown-unknownunknown
vhl-1Caenorhabditis elegans181321deletion / nullok161unknownunknown
increased 150.25% 49.3 19.7 days mean 20 hermaphrodite RNAi knock-down of daf-2 increased life span additively with deletion of vhl-1. These data support the model that VHL-1 and insulin-like signaling act in parallel pathways to modulate longevity. Mehta et al. 2009 19372390 [DOI icon]doi:10.1126/science.1173507 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 1018)
137 OB_002110 Caenorhabditis elegans
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
let-363Caenorhabditis elegans172167RNAi knockdown-unknownunknown
increased 150.00% RNA interference extends lifespan by about 150%. Vellai et al. 2003 14668850 [DOI icon]doi:10.1038/426620a [Lifespan Observations Database icon]Lifespan Observations Database (ID: 339)
138 OB_002317 Caenorhabditis elegans N2
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
daf-2Caenorhabditis elegans175410loss of functione1370unknownunknown
increased 150.00% 44 17.6 days mean 20 hermaphrodite Mutatation extended lifespan by 150%. Oh et al. 2005 15767565 [DOI icon]doi:10.1073/pnas.0500749102 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 3592)
139 OB_005672 Caenorhabditis elegans
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
osm-5Caenorhabditis elegans180585Mutation-wildtypeyes
increased 150.00% unknown Loss-of-function mutation can increase lifespan by 100-150%. Apfeld et al. 1999 10617200 [DOI icon]doi:10.1038/45544 [GenAge icon]GenAge
140 OB_006135 Caenorhabditis elegans
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
unc-13Caenorhabditis elegans172497Mutation-wildtypeyes
increased 150.00% unknown Mutation results in a 150% life-extension in males. Gems et al. 2000 10747056 [GenAge icon]GenAge
141 OB_006138 Caenorhabditis elegans
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
unc-31Caenorhabditis elegans178233Mutation-wildtypeyes
increased 150.00% unknown Mutation increases hermaphrodite lifespan by approximately 70% and male lifespan by 150%. Ailion et al. 1999 10377425 [GenAge icon]GenAge
142 OB_007868 Caenorhabditis elegans
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
let-363Caenorhabditis elegans172167RNA interference-wildtypeyes
increased 150.00% unknown RNA interference extends lifespan by about 150%. Vellai et al. 2003 14668850 [DOI icon]doi:10.1038/426620a [GenAge icon]GenAge
143 OB_001791 Caenorhabditis elegans N2
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
daf-2Caenorhabditis elegans175410loss of functione1370unknownunknown
increased 149.66% 36.7 14.7 days median 25 hermaphrodite A genome-wide RNAi screen was performed to identify genes necessary for the extended life span of daf-2 mutants and identified approximately 200 gene inactivations that shorten daf-2 life span. Some of these gene inactivations dramatically shorten daf-2 mutant life span but less dramatically shorten daf-2; daf-16 mutant or wild-type life span. RNAi experiments were initiated at egg. Animals were grown at 15 degrees C until L4, then transferred to 25 degrees C. Samuelson et al. 2007 18006689 [DOI icon]doi:10.1101/gad.1588907 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 3111)
144 OB_001476 Caenorhabditis elegans N2
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
daf-2Caenorhabditis elegans175410unknowne1370unknownunknown
increased 149.00% 62 24.9 days mean 20 hermaphrodite Treatment of Caenorhabditis elegans with trehalose starting from the young-adult stage extended the mean life span by over 30% without any obvious side effects. Trehalose extended life span more effectively on UV-killed E. coli than on live E. coli. Trehalose also enhanced thermotolerance and reduced polyglutamine aggregation. The life span-extending effect of trehalose was abolished in long-lived insulin/IGF-1-like receptor (daf-2) mutants. RNA interference-mediated inactivation of the trehalose-biosynthesis genes trehalose-6-phosphate synthase-1 (tps-1) and tps-2, which are known to be up-regulated in daf-2 mutants, decreased the daf-2 life span. Honda et al. 2010 20477758 [DOI icon]doi:10.1111/j.1474-9726.2010.00582.x [Lifespan Observations Database icon]Lifespan Observations Database (ID: 2798)
145 OB_001219 Caenorhabditis elegans N2
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
daf-2Caenorhabditis elegans175410loss of function-unknownunknown
gpa-11Caenorhabditis elegans173759over-expression-unknownunknown
odr-3Caenorhabditis elegans179806loss of function-unknownunknown
increased 147.22% 44.5 18 days mean hermaphrodite Loss of function mutation of daf-2 and odr-3 and over-expression of gpa-11 increased lifespan 147%. Lans et al. 2007 17187771 [DOI icon]doi:10.1016/j.ydbio.2006.11.028 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 2542)
146 OB_003579 Caenorhabditis elegans N2
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
daf-2Caenorhabditis elegans175410loss of functione1370unknownunknown
trx-1Caenorhabditis elegans181863loss of functionok1449unknownunknown
increased 146.67% 37 15 days max 20 Maximum lifespan of trx-1(ok1449);daf-2(e1370) double mutant was approximately 150% longer than control. Fierro-González et al. 2011 21334311 [DOI icon]doi:10.1016/j.bbrc.2011.02.079 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 5013)
147 OB_001024 Caenorhabditis elegans N2
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
daf-2Caenorhabditis elegans175410loss of functione1370unknownunknown
sir-2.1Caenorhabditis elegans177924over-expressiongeIn3unknownunknown
increased 145.90% 45 18.3 days mean 20 hermaphrodite Transgenic overexpression of sir-2.1 from an extrachromosomal or integrated multicopy array extends life span. Other phenotypes: Life span extension is suppressed by a mutation in daf-16 and does not synergize with daf-2. Overexpression of sir-2.1 synergizes with the TGF-beta mutations daf-4 and daf-1 for dauer formation. Tissenbaum et al. 2001 11242085 [DOI icon]doi:10.1038/35065638 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 2345)
148 OB_001699 Caenorhabditis elegans N2
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
daf-2Caenorhabditis elegans175410unknownm579unknownunknown
increased 145.40% 40 16.3 days median 22.5 hermaphrodite 15 temperature-sensitive and 1 nonconditional daf-2 allele were characterized to investigate the extent of daf-2 mutant defects and to examine whether specific mutant traits correlate with each other. The greatest longevity seen in daf-2 mutant adults was approximately three times that of wild type. The temperature-sensitive daf-2 mutants fell into two overlapping classes, including eight class 1 mutants, which are Daf-c, Age, and Itt, and exhibit low levels of L1 arrest at 25.5 degrees. Seven class 2 mutants also exhibit the class 1 defects as well as some or all of the following: reduced adult motility, abnormal adult body and gonad morphology, high levels of embryonic and L1 arrest, production of progeny late in life, and reduced brood size. The strengths of the Daf-c, Age, and Itt phenotypes largely correlated with each other but not with the strength of class 2-specific defects. This suggests that the DAF-2 receptor is bifunctional. Gems et al. 1998 9725835 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 3029)
149 OB_000918 Caenorhabditis elegans N2
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
daf-12Caenorhabditis elegans181263unknownm20unknownunknown
daf-2Caenorhabditis elegans175410unknowne1391unknownunknown
increased 144.58% 58.7 24 days median 15 hermaphrodite 15 temperature-sensitive and 1 nonconditional daf-2 allele were characterized to investigate the extent of daf-2 mutant defects and to examine whether specific mutant traits correlate with each other. The greatest longevity seen in daf-2 mutant adults was approximately three times that of wild type. The temperature-sensitive daf-2 mutants fell into two overlapping classes, including eight class 1 mutants, which are Daf-c, Age, and Itt, and exhibit low levels of L1 arrest at 25.5 degrees. Seven class 2 mutants also exhibit the class 1 defects as well as some or all of the following: reduced adult motility, abnormal adult body and gonad morphology, high levels of embryonic and L1 arrest, production of progeny late in life, and reduced brood size. The strengths of the Daf-c, Age, and Itt phenotypes largely correlated with each other but not with the strength of class 2-specific defects. This suggests that the DAF-2 receptor is bifunctional. Gems et al. 1998 9725835 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 2225)
150 OB_001686 Caenorhabditis elegans N2
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
daf-2Caenorhabditis elegans175410unknownsa223unknownunknown
increased 144.58% 58.7 24 days median 15 hermaphrodite 15 temperature-sensitive and 1 nonconditional daf-2 allele were characterized to investigate the extent of daf-2 mutant defects and to examine whether specific mutant traits correlate with each other. The greatest longevity seen in daf-2 mutant adults was approximately three times that of wild type. The temperature-sensitive daf-2 mutants fell into two overlapping classes, including eight class 1 mutants, which are Daf-c, Age, and Itt, and exhibit low levels of L1 arrest at 25.5 degrees. Seven class 2 mutants also exhibit the class 1 defects as well as some or all of the following: reduced adult motility, abnormal adult body and gonad morphology, high levels of embryonic and L1 arrest, production of progeny late in life, and reduced brood size. The strengths of the Daf-c, Age, and Itt phenotypes largely correlated with each other but not with the strength of class 2-specific defects. This suggests that the DAF-2 receptor is bifunctional. Gems et al. 1998 9725835 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 3017)
151 OB_001023 Caenorhabditis elegans N2
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
daf-2Caenorhabditis elegans175410loss of functione1370unknownunknown
increased 144.26% 44.7 18.3 days mean 20 hermaphrodite Transgenic overexpression of sir-2.1 from an extrachromosomal or integrated multicopy array extends life span. Other phenotypes: Life span extension is suppressed by a mutation in daf-16 and does not synergize with daf-2. Overexpression of sir-2.1 synergizes with the TGF-beta mutations daf-4 and daf-1 for dauer formation. Tissenbaum et al. 2001 11242085 [DOI icon]doi:10.1038/35065638 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 2344)
152 OB_003086 Saccharomyces cerevisiae YPK9
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
RPD3Saccharomyces cerevisiae855386deletion / null-unknownunknown
Other Ageing Factor NameAdditional Information
Dietary restrictionNonessential amino acids removed from media.
increased 142.86% 28.9 11.9 divisions mean 30 mata Combining deletion of the histone deacetylase RPD3 with dietary restriction by removal of nonessential amino acids from the media increases lifespan by 142.9%, an extension similar to that imparted by amino acid restriction alone. Jiang et al. 2002 12213553 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 4461)
153 OB_002344 Caenorhabditis elegans N2
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
daf-2Caenorhabditis elegans175410loss of functione1370unknownunknown
increased 141.21% 43.9 18.2 days mean 20 hermaphrodite Mutation extended lifespan by approximately 140%. Berman et al. 2006 16530050 [DOI icon]doi:10.1016/j.cell.2006.01.039 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 3616)
154 OB_008101 Caenorhabditis elegans N2
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
daf-2Caenorhabditis elegans175410RNAi knockdown-wildtypeyes
increased 141.00% 46.76 19.4 day mean hermaphrodite Zarse et al. 2012 22482728 [DOI icon]doi:10.1016/j.cmet.2012.02.013 [AgeFactDB Curator icon]AgeFactDB Curator
155 OB_000869 Saccharomyces cerevisiae W303AR5
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
SIR2Saccharomyces cerevisiae851520deletion / null-unknownunknown
Other Ageing Factor NameAdditional Information
Dietary restrictionReduced glucose in the medium to 0.5%
increased 140.00% 12 5 days median 30 mata Deletion of sir2, hst1, hst2, hst3, hst4 or all 5 sirtuins at the same time had no effect on chronological life span and did not reduce life span extension from dietary restriction at 0.5% or 0.05% glucose. Murakami et al. 2008 18314444 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 2174)
156 OB_000870 Saccharomyces cerevisiae W303AR5
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
SIR2Saccharomyces cerevisiae851520deletion / null-unknownunknown
Other Ageing Factor NameAdditional Information
Dietary restrictionReduced glucose in the medium to 0.05%
increased 140.00% 12 5 days median 30 mata Deletion of sir2, hst1, hst2, hst3, hst4 or all 5 sirtuins at the same time had no effect on chronological life span and did not reduce life span extension from dietary restriction at 0.5% or 0.05% glucose. Murakami et al. 2008 18314444 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 2175)
157 OB_003225 Caenorhabditis elegans N2
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
sod-3Caenorhabditis elegans181748deletion / null-unknownunknown
Compound NameQuantityQuantity Unit
Paraquat200mM
increased 140.00% 600 250 mean 25 In an acute survival test using 200 mM paraquat solution, sod-1 worms were hypersensitive to paraquat, whereas, surprisingly, sod-3 or sod-4 worms actually had a reduced sensitivity to paraquat. Mutational inactivation of other isoforms of sod had no effect on sensitivity to paraquat in this acute assay. Yen et al. 2009 19059428 [DOI icon]doi:10.1016/j.mad.2008.11.003 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 4634)
158 OB_004821 Saccharomyces cerevisiae W303AR5
Other Ageing Factor NameAdditional Information
Dietary restrictionReduced glucose in the medium to 0.5%
increased 140.00% 12 5 days median 30 mata Dietary restriction by reducing the glucose concentration of the medium from 2% to 0.5% or 0.05% increased chronological life span in the BY4742, PSY316AR, and W303AR5 strain backgrounds. Murakami et al. 2008 18314444 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 2166)
159 OB_004822 Saccharomyces cerevisiae W303AR5
Other Ageing Factor NameAdditional Information
Dietary restrictionReduced glucose in the medium to 0.05%
increased 140.00% 12 5 days median 30 mata Dietary restriction by reducing the glucose concentration of the medium from 2% to 0.5% or 0.05% increased chronological life span in the BY4742, PSY316AR, and W303AR5 strain backgrounds. Murakami et al. 2008 18314444 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 2167)
160 OB_008093 Caenorhabditis elegans N2
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
daf-2Caenorhabditis elegans175410RNAi knockdown-wildtypeyes
increased 140.00% 72 30 day maximum hermaphrodite Zarse et al. 2012 22482728 [DOI icon]doi:10.1016/j.cmet.2012.02.013 [AgeFactDB Curator icon]AgeFactDB Curator
161 OB_004915 Saccharomyces cerevisiae YPK9
Other Ageing Factor NameAdditional Information
Dietary restrictionNonessential amino acids removed from media.
increased 139.50% 28.5 11.9 divisions mean 30 mata Dietary restriction by removal of nonessential amino acids from the media extends lifespan by 139.5%. Jiang et al. 2002 12213553 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 4460)
162 OB_000851 Caenorhabditis elegans N2
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
che-3Caenorhabditis elegans172593unknownp801unknownunknown
Other Ageing Factor NameAdditional Information
Dietary restrictionBacterial deprivation beginning at the 4th day of adulthood.
increased 139.13% 55 23 days median 20 hermaphrodite Dietary restriction by bacterial deprivation significantly increased the life span of long-lived mutants defective for chemosensary function. Smith et al. 2008 18457595 [DOI icon]doi:10.1186/1471-213X-8-49 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 2132)
163 OB_000855 Caenorhabditis elegans N2
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
daf-10Caenorhabditis elegans184883unknowne1387unknownunknown
Other Ageing Factor NameAdditional Information
Dietary restrictionBacterial deprivation beginning at the 4th day of adulthood.
increased 139.13% 55 23 days median 20 hermaphrodite Dietary restriction by bacterial deprivation significantly increased the life span of long-lived mutants defective for chemosensary function. Smith et al. 2008 18457595 [DOI icon]doi:10.1186/1471-213X-8-49 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 2137)
164 OB_003026 Saccharomyces cerevisiae
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
GLN3Saccharomyces cerevisiae856763deletion / null-unknownunknown
increased 138.00% si Deletion extends chronological lifespan approximately 138%. Powers et al. 2006 16418483 [DOI icon]doi:10.1101/gad.1381406 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 4372)
165 OB_000814 Caenorhabditis elegans N2
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
daf-2Caenorhabditis elegans175410loss of functione1370unknownunknown
Other Ageing Factor NameAdditional Information
Q-less dietFeeding worms GD1, an E. coli strain unable to synthesize coenzyme Q beginnign at L4.
increased 137.50% 38 16 days median 25 hermaphrodite Feeding C. elegans the E. coli strain GD1, which is unable to synthesize coenzyme Q, increases life span relative to animals fed the standard E. coli strain OP50.Life span extension from GD1 also occurred in the following mutant backgrounds: daf-12(m20), daf-16(m26), clk-1(e2519), clk-1(qm30), clk-1(qm51), daf-2(e1370); clk-1(qm30) and daf-2(e1370); daf-12(m20)It should be noted that GD1 is a different strain background than OP50 Larsen et al. 2002 11778046 [DOI icon]doi:10.1126/science.1064653 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 2076)
166 OB_002606 Caenorhabditis elegans N2
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
daf-2Caenorhabditis elegans175410loss of functione1370unknownunknown
Compound NameQuantityQuantity Unit
Ethosuximide4mg/ml
increased 135.33% 39.3 16.7 days mean 20 hermaphrodite The anti-convulsant compounds ethosuximide, trimethadione, and 3,3-diethyl-2-pyrrolidinone increase mean and maximum life-span of Caenorhabditis elegans and delay age-related declines of physiological processes. Evason et al. 2005 15653505 [DOI icon]doi:10.1126/science.1105299 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 3892)
167 OB_002518 Caenorhabditis elegans N2
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
daf-2Caenorhabditis elegans175410loss of functione1370unknownunknown
pept-1Caenorhabditis elegans180919deletion / nulllg601unknownunknown
increased 134.56% 31.9 13.6 days mean 20 hermaphrodite Deletion of opt-2, also called pep-2, extends (60%) the life-extension caused by daf-2 mutations.Other phenotypes: Also results in retarded development, reduced body size and extended reproductive lifespan. Meissner et al. 2004 15155758 [DOI icon]doi:10.1074/jbc.M403415200 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 3773)
168 OB_001695 Caenorhabditis elegans N2
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
daf-2Caenorhabditis elegans175410unknowne1369unknownunknown
increased 134.36% 38.2 16.3 days median 22.5 hermaphrodite 15 temperature-sensitive and 1 nonconditional daf-2 allele were characterized to investigate the extent of daf-2 mutant defects and to examine whether specific mutant traits correlate with each other. The greatest longevity seen in daf-2 mutant adults was approximately three times that of wild type. The temperature-sensitive daf-2 mutants fell into two overlapping classes, including eight class 1 mutants, which are Daf-c, Age, and Itt, and exhibit low levels of L1 arrest at 25.5 degrees. Seven class 2 mutants also exhibit the class 1 defects as well as some or all of the following: reduced adult motility, abnormal adult body and gonad morphology, high levels of embryonic and L1 arrest, production of progeny late in life, and reduced brood size. The strengths of the Daf-c, Age, and Itt phenotypes largely correlated with each other but not with the strength of class 2-specific defects. This suggests that the DAF-2 receptor is bifunctional. Gems et al. 1998 9725835 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 3025)
169 OB_002689 Caenorhabditis elegans N2
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
eat-2Caenorhabditis elegans175072loss of functionad1116unknownunknown
nuo-6Caenorhabditis elegans172438loss of functionqm200unknownunknown
increased 134.13% 48.7 20.8 days mean 20 hermaphrodite Mutation of nuo-6 or isp-1 appears to increase life span by a mechanism that is genetically distinct from RNAi of these genes. Yang et al. 2010 20346072 [DOI icon]doi:10.1111/j.1474-9726.2010.00571.x [Lifespan Observations Database icon]Lifespan Observations Database (ID: 3984)
170 OB_002961 Caenorhabditis elegans N2
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
daf-2Caenorhabditis elegans175410non-null recessivee1370unknownunknown
increased 133.59% 30.6 13.1 days mean 20 hermaphrodite In Caenorhabditis elegans, functional ttx-3, sra-11, ceh-10, and ceh-23 genes are required for the functions of AIY interneuron. Compared to wild-type N2, mutations in ttx-3 and ceh-10 significantly decreased lifespan, whereas mutations in sra-11 and ceh-23 did not obviously influence nematode lifespan. Mutations in ttx-3 and ceh-10 were associated closely with lower pumping rates at adult day 8 and caused a more rapid accumulated intestinal autofluorescence than wild-type N2. Mutations in ceh-10 remarkably affected fertility and egg number in the uterus. The regulation of ttx-3 and ceh-10 on longevity was not temperature-dependent, and ttx-3, and ceh-10 mutants all formed very few dauers at 27 C. The shortened lifespan of the ttx-3 or ceh-10 mutants was completely or largely rescued by expression of TTX-3 or CEH-10 in AIY interneurons. Moreover, the long-lived phenotype of the daf-2 mutant could be suppressed by both the ttx-3 and the ceh-10 mutations. Furthermore, ablation of AIY interneurons shortened the longevity of wild-type and the daf-2 mutant. Shen et al. 2010 21055415 [DOI icon]doi:10.1016/j.mad.2010.10.005 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 4280)
171 OB_001350 Saccharomyces cerevisiae BY4741
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
aro7Saccharomyces cerevisiaedeletion / null-unknownunknown
increased 133.33% 35 15 days median 30 mata A screen of a complete set of approximately 4,800 viable deletion mutants was performed to identify genes that either increase or decrease chronological life span. Half of the putative short-/long-lived mutants retested from the primary screen were confirmed, demonstrating the utility of our approach. Deletion of genes involved in vacuolar protein sorting, autophagy, and mitochondrial function shortened life span, confirming that respiration and degradation processes are essential for long-term survival. Among the genes whose deletion significantly extended life span are ACB1, CKA2, and TRM9, implicated in fatty acid transport and biosynthesis, cell signaling, and tRNA methylation, respectively. Fabrizio et al. 2010 20657825 [DOI icon]doi:10.1371/journal.pgen.1001024 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 2669)
172 OB_003074 Saccharomyces cerevisiae X2180-1A
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
X2180-1BSaccharomyces cerevisiaeunknown-unknownunknown
increased 133.33% 28 12 divisions mean 30 mata Two strains with different lifespan characteristics (X2180-1A is shorter lived than X2180-1B) were crossed to form a diploid strain. The diploid strain had lifespan similar to X2180-1B, the longer lived of the two haploid parents. Jazwinski et al. 1989 2698814 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 4445)
173 OB_003563 Caenorhabditis elegans N2
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
daf-2Caenorhabditis elegans175410unknownsa189unknownunknown
increased 133.33% 42 18 days mean 20 hermaphrodite Mutation of daf-2 increases life span. Animals were shifted from 15 degress to 20 degrees C at L4. Kenyon et al. 1993 8247153 [DOI icon]doi:10.1038/366461a0 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 49)
174 OB_004740 Saccharomyces cerevisiae PSY316AUT
Other Ageing Factor NameAdditional Information
Dietary restriction0.05% glucose
rho0Absence of mitochondrial DNA.
increased 133.33% 28 12 divisions mean 30 matalpha Dietary restriction at either 0.5% or 0.05% glucose increases the replicative life span of rho0 cells in the PSY316AUT strain background, demonstrating that mitochondrial respiration is not required for life span extension from dietary restriction. Kaeberlein et al. 2005 16311627 [DOI icon]doi:10.1371/journal.pgen.0010069 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 1115)
175 OB_003431 Caenorhabditis elegans N2
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
cdc-25.1Caenorhabditis elegans172353RNAi knockdown-unknownunknown
sgk-1Caenorhabditis elegans181697loss of functionok538unknownunknown
Other Ageing Factor NameAdditional Information
UV-killed dietOP50-1 was grown in LB with 100 mg mL1 streptomycin, concentrated 20, and 80 L was spread onto NG plates. Plates were placed at ~23 C for 16 h then exposed to UV in a Stratagene Stratalinker 2400 for 4 min.
increased 132.67% 606.8 260.8 mean 25 Lifespan of sgk-1(ok538) mutant on UV-killed OP50 was approximately 132% longer than N2 control on cdc-25.1 RNAi background. Evans et al. 2008 18782349 [DOI icon]doi:10.1111/j.1474-9726.2008.00435.x [Lifespan Observations Database icon]Lifespan Observations Database (ID: 4855)
176 OB_002341 Caenorhabditis elegans N2
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
daf-2Caenorhabditis elegans175410loss of functione1370unknownunknown
increased 130.46% 45.4 19.7 days mean 20 hermaphrodite Mutation extended lifespan by approximately 130%. Berman et al. 2006 16530050 [DOI icon]doi:10.1016/j.cell.2006.01.039 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 3613)
177 OB_005017 Saccharomyces cerevisiae BY4742
Other Ageing Factor NameAdditional Information
Dietary restrictionReducing the glucose concentration of the medium from 2% to 0.5%.
increased 130.00% 23 10 days mean 30 matalpha Reducing the glucose concentration of the synthetic defined culture medium from 2% to 0.5% significantly increase yeast chronological life span. Goldberg et al. 2010 20622262 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 931)
178 OB_002688 Caenorhabditis elegans N2
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
isp-1Caenorhabditis elegans177609RNAi knockdown25% RNAiunknownunknown
nuo-6Caenorhabditis elegans172438loss of functionqm200unknownunknown
increased 129.63% 49.6 21.6 days mean 20 hermaphrodite Mutation of nuo-6 or isp-1 appears to increase life span by a mechanism that is genetically distinct from RNAi of these genes. Yang et al. 2010 20346072 [DOI icon]doi:10.1111/j.1474-9726.2010.00571.x [Lifespan Observations Database icon]Lifespan Observations Database (ID: 3983)
179 OB_001025 Caenorhabditis elegans N2
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
daf-2Caenorhabditis elegans175410loss of functione1370unknownunknown
sir-2.1Caenorhabditis elegans177924over-expressiongeEx1unknownunknown
increased 128.96% 41.9 18.3 days mean 20 hermaphrodite Transgenic overexpression of sir-2.1 from an extrachromosomal or integrated multicopy array extends life span. Other phenotypes: Life span extension is suppressed by a mutation in daf-16 and does not synergize with daf-2. Overexpression of sir-2.1 synergizes with the TGF-beta mutations daf-4 and daf-1 for dauer formation. Tissenbaum et al. 2001 11242085 [DOI icon]doi:10.1038/35065638 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 2346)
180 OB_000865 Saccharomyces cerevisiae BY4742
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
SIR2Saccharomyces cerevisiae851520deletion / null-unknownunknown
Other Ageing Factor NameAdditional Information
Dietary restrictionReduced glucose in the medium to 0.5%
increased 128.57% 16 7 days median 30 matalpha Deletion of sir2, hst1, hst2, hst3, hst4 or all 5 sirtuins at the same time had no effect on chronological life span and did not reduce life span extension from dietary restriction at 0.5% or 0.05% glucose. Murakami et al. 2008 18314444 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 2170)
181 OB_004817 Saccharomyces cerevisiae BY4742
Other Ageing Factor NameAdditional Information
Dietary restrictionReduced glucose in the medium to 0.5%
increased 128.57% 16 7 days median 30 matalpha Dietary restriction by reducing the glucose concentration of the medium from 2% to 0.5% or 0.05% increased chronological life span in the BY4742, PSY316AR, and W303AR5 strain backgrounds. Murakami et al. 2008 18314444 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 2162)
182 OB_001117 Caenorhabditis elegans N2
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
osm-5Caenorhabditis elegans180585unknownp813unknownunknown
increased 127.72% 41.9 18.4 days mean 20 hermaphrodite Several (but not all) mutations that cause defects in sensory cilia or their support cells, or in sensory signal transduction, extend lifespan. These mutations do not further extend long-lived daf-2 mutants Apfeld et al. 1999 10617200 [DOI icon]doi:10.1038/45544 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 2437)
183 OB_002557 Caenorhabditis elegans N2
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
daf-2Caenorhabditis elegans175410deletion / nulle1370unknownunknown
increased 127.14% 31.8 14 days mean 25 hermaphrodite Mutation extends lifespan approximately 127%. Mukhopadhyay et al. 2005 16054097 [DOI icon]doi:10.1016/j.cmet.2005.06.004 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 3808)
184 OB_002237 Caenorhabditis elegans N2
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
daf-2Caenorhabditis elegans175410loss of function(e1370)IIIunknownunknown
gpi-1Caenorhabditis elegans173227RNAi knockdown-unknownunknown
increased 126.97% 34.5 15.2 days mean 25 hermaphrodite For calculating relative effect of lifespan in mutant background, an average of all N2 data in Supplemental Table I was used since no experiment matched control was provided. RNA knockdown increased lifespan of daf-2 mutants by 38% relative to daf-2 mutant lifespan alone. Hansen et al. 2005 16103914 [DOI icon]doi:10.1371/journal.pgen.0010017 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 3513)
185 OB_002554 Caenorhabditis elegans N2
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
daf-2Caenorhabditis elegans175410deletion / nulle1370unknownunknown
tub-1Caenorhabditis elegans174312deletion / nullnr2004unknownunknown
increased 126.43% 31.7 14 days mean 25 hermaphrodite Double mutation extends lifespan 125%. Mukhopadhyay et al. 2005 16054097 [DOI icon]doi:10.1016/j.cmet.2005.06.004 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 3805)
186 OB_003001 Caenorhabditis elegans N2
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
daf-16Caenorhabditis elegans172981unknownm26unknownunknown
Other Ageing Factor NameAdditional Information
Axenic mediumCulture on non-bacterial, semi-dened, axenic food sources.
increased 126.39% 32.6 14.4 days mean 24 hermaphrodite Comparisons of longevity of wild type and mutants grown on bacteria vs. axenic culture. Houthoofd et al. 2003 12954481 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 4335)
187 OB_003497 Caenorhabditis elegans N2DRM
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
daf-2Caenorhabditis elegans175410loss of functione1370unknownunknown
increased 126.00% median 20 Median lifespan of strain SR806 daf-2(e1370) was approximately 126% longer than N2DRM control. Shmookler Reis et al. 2011 21386131 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 4925)
188 OB_003950 Caenorhabditis elegans rrf-3(pk1426)
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
rps-23Caenorhabditis elegans178188RNAi knockdown-unknownunknown
increased 126.00% RNA interference in the daf-16(+) background increased mean lifespan by 126%. Kim et al. 2007 17608836 [DOI icon]doi:10.1111/j.1474-9726.2007.00302.x [Lifespan Observations Database icon]Lifespan Observations Database (ID: 661)
189 OB_005843 Caenorhabditis elegans
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
rps-23Caenorhabditis elegans178188RNA interference-wildtypeyes
increased 126.00% Average RNA interference increased mean lifespan by 126%. Kim et al. 2007 17608836 [DOI icon]doi:10.1111/j.1474-9726.2007.00302.x [GenAge icon]GenAge
190 OB_002607 Caenorhabditis elegans N2
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
daf-2Caenorhabditis elegans175410loss of functione1370unknownunknown
Compound NameQuantityQuantity Unit
Trimethadione4mg/ml
increased 125.15% 37.6 16.7 days mean 20 hermaphrodite The anti-convulsant compounds ethosuximide, trimethadione, and 3,3-diethyl-2-pyrrolidinone increase mean and maximum life-span of Caenorhabditis elegans and delay age-related declines of physiological processes. Evason et al. 2005 15653505 [DOI icon]doi:10.1126/science.1105299 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 3893)
191 OB_000821 Caenorhabditis elegans N2
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
daf-12Caenorhabditis elegans181263loss of functionm20unknownunknown
daf-2Caenorhabditis elegans175410normale1370unknownunknown
increased 125.00% 36 16 days median 25 hermaphrodite Feeding C. elegans the E. coli strain GD1, which is unable to synthesize coenzyme Q, increases life span relative to animals fed the standard E. coli strain OP50.Life span extension from GD1 also occurred in the following mutant backgrounds: daf-12(m20), daf-16(m26), clk-1(e2519), clk-1(qm30), clk-1(qm51), daf-2(e1370); clk-1(qm30) and daf-2(e1370); daf-12(m20)It should be noted that GD1 is a different strain background than OP50 Larsen et al. 2002 11778046 [DOI icon]doi:10.1126/science.1064653 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 2082)
192 OB_002139 Caenorhabditis elegans N2
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
daf-2Caenorhabditis elegans175410loss of function(e1370)IIIunknownunknown
drr-2Caenorhabditis elegans259546RNAi knockdown-unknownunknown
increased 124.48% 43.1 19.2 days mean 20 hermaphrodite For calculating relative effect of lifespan in mutant background, an average of all N2 data in Supplemental Table I was used since no experiment matched control was provided. RNA knockdown had no significant effect on lifespan of daf-2 mutants. Hansen et al. 2005 16103914 [DOI icon]doi:10.1371/journal.pgen.0010017 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 3425)
193 OB_003315 Caenorhabditis elegans N2
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
age-1Caenorhabditis elegans174762loss of functionmg305unknownunknown
increased 123.81% 18.8 8.4 mean 35 Mean lifespan of age-1(mg305) mutant was 124% greater than wild type under heat stress. Houthoofd et al. 2005 15771612 [DOI icon]doi:10.1111/j.1474-9726.2005.00150.x [Lifespan Observations Database icon]Lifespan Observations Database (ID: 4739)
194 OB_002644 Caenorhabditis elegans N2
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
daf-2Caenorhabditis elegans175410loss of functione1370unknownunknown
Compound NameQuantityQuantity Unit
Valproic acid6mM
increased 123.46% 36.2 16.2 days mean 20 hermaphrodite The mood stabilizer and anticonvulsant valproic acid (VA) extended C. elegans lifespan. Evason et al. 2008 18248662 [DOI icon]doi:10.1111/j.1474-9726.2008.00375.x [Lifespan Observations Database icon]Lifespan Observations Database (ID: 3937)
195 OB_003166 Caenorhabditis elegans N2
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
daf-2Caenorhabditis elegans175410loss of functione1370unknownunknown
Other Ageing Factor NameAdditional Information
Caloric restrictionComplete absence of bacterial food.
increased 123.08% 58 26 days median 20 DR-FD does not require daf-16 and is additive with daf-2. DR-FD further increases the long lifespan of daf-2(e1370) mutants. Median lifespans: N2 Fed, 19 days; N2 DR-FD, 26 days; daf-2 Fed, 39 days; daf-2 DR-FD, 58 days. DR of daf-2 mutant is extended by approximately 123% relative to DR of N2. Kaeberlein et al. 2006 17081160 [DOI icon]doi:10.1111/j.1474-9726.2006.00238.x [Lifespan Observations Database icon]Lifespan Observations Database (ID: 4562)
196 OB_004948 Caenorhabditis elegans sir-2(ok434)
Other Ageing Factor NameAdditional Information
Dietary deprivationAdult worms transferred to plates without food.
increased 122.00% days max DD initiated at adult day 2 extended both mean and maximum lifespan in sir-2.1(ok434) worms by 81.8% (P < 0.0001) and 121.8% (P < 0.0001), respectively, compared to AL. Lee et al. 2006 17096674 [DOI icon]doi:10.1111/j.1474-9726.2006.00241.x [Lifespan Observations Database icon]Lifespan Observations Database (ID: 4581)
197 OB_002707 Caenorhabditis elegans N2
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
osm-5Caenorhabditis elegans180585unknownp802unknownunknown
increased 121.28% 41.6 18.8 days mean 20 hermaphrodite Several (but not all) mutations that cause defects in sensory cilia or their support cells, or in sensory signal transduction, extend lifespan. Apfeld et al. 1999 10617200 [DOI icon]doi:10.1038/45544 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 3)
198 OB_001214 Caenorhabditis elegans N2
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
daf-2Caenorhabditis elegans175410loss of function-unknownunknown
increased 121.11% 39.8 18 days mean hermaphrodite Loss of function mutation of increased lifespan 120%. Lans et al. 2007 17187771 [DOI icon]doi:10.1016/j.ydbio.2006.11.028 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 2538)
199 OB_000443 Caenorhabditis elegans N2
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
daf-2Caenorhabditis elegans175410unknowne1370unknownunknown
increased 121.05% 42 19 days mean 20 hermaphrodite Mutations in daf-16 suppress life-extension caused by mutations in daf-2. Kenyon et al. 1993 8247153 [DOI icon]doi:10.1038/366461a0 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 170)
200 OB_002524 Caenorhabditis elegans N2
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
daf-2Caenorhabditis elegans175410loss of functione1370unknownunknown
increased 120.91% 24.3 11 days mean 25 hermaphrodite Mutation extends lifespan approximately 120%. Meissner et al. 2004 15155758 [DOI icon]doi:10.1074/jbc.M403415200 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 3779)