Research Centre Information Centre AgeFactDB
Release 1 - Aug 15, 2013

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Ageing Phenotype Observations List—Data Type 2

NOTE:

We distinguish between different types of ageing relevance evidence. Experimental studies on ageing factors result in observations with experimental ageing relevance evidence (1). According to our definition of ageing factors a comparison of two situations, with and without the action of the ageing factor, is required. Almost all data in AgeFactDB is of this type. There are however also a few lifespan data for populations or species included that are certainly relevant to ageing with a slightly different meaning of the term ageing relevance, however. But in this case there is no intervention study and therefore these data cannot be assigned to an ageing factor (2). Furthermore, there are experimental studies where no significant effect of an assumed ageing factor was found (3). And finally there are genes included that are homologous to known ageing-relevant genes for which there is no experimental evidence. They are considered as putative ageing-relevant genes with computational evidence (4).
Order of importance:

  1. yes (Experimental Analysis)
  2. yes, but no ageing factor assigned (Experimental Analysis)
  3. no (Experimental Analysis)
  4. putative (Computational Analysis)
The color indicating the ageing relevance type of an observation may differ from the corresponding color for the ageing factor related to this observation. The reason is that for ageing factors associated with more than one observation, the most important observation color is chosen as ageing factor color. This is always the color with the lowest number in the order given above.

Ageing phenotype observations are considered as experimental analysis. They confirm or reject the ageing relevance for particular ageing factor(s). For genes that are homologous to known ageing-relevant genes there is thus far no experimental evidence. They are considered as candidate genes.
Due to the different data structures of ageing phenotype observation data integrated from other databases we subdivide these observations into type 1 and 2. Observations of type 1 contain the phenotype data mostly unseparated within a single description. Observations of type 2 contain lifespan data in a more structured form (e.g.: separated into lifespan effect, lifespan change, lifespan value). Currently there might be an overlap between the observations of type 1 and 2 from the same source, namely GenAge. But we are working on a reduction of this overlap.

Ageing Relevance:
1 ... 100 [next icon]
# Observation Stable ID Species Strain Gene Compound Other Ageing Factor Significant Lifespan Effect Change Observed Lifespan Reference Lifespan Lifespan Unit Measure Temperature Temperature Unit Sex/Mating Type Description Author Year PubMed DOI Source
1 OB_004896 Podospora anserina s
Other Ageing Factor NameAdditional Information
Dietary restrictionReducing glucose from 2% to 0.02%.
increased 1370.59% 250 17 days mean Dietary restriction by reducing availability of the carbon source increases life span in P. anserina. van Diepeningen et al. 2010 20026344 [DOI icon]doi:10.1016/j.mad.2009.12.002 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 4286)
2 OB_004905 Podospora anserina Wa32
Other Ageing Factor NameAdditional Information
Dietary restrictionReducing glucose from 2% to 0.02%.
increased 1333.33% 258 18 days mean Dietary restriction by reducing availability of the carbon source increases life span in P. anserina. van Diepeningen et al. 2010 20026344 [DOI icon]doi:10.1016/j.mad.2009.12.002 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 4295)
3 OB_002655 Escherichia coli BW25113
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
lpdAEscherichia colideletion / null-unknownunknown
increased 1166.67% 19 1.5 days median 37 E. coli strains lacking tricarboxylic acid (TCA)-cycle-related genes exhibit longer stationary-phase survival and increased resistance to heat stress compared to wild-type. Extended lifespan in the sdhA mutant, lacking subunit A of succinate dehydrogenase, is associated with the reduced production of superoxide and increased stress resistance. On the other hand, the longer lifespan of the lipoic acid synthase mutant (lipA) is associated with reduced oxygen consumption and requires the acetate-producing enzyme pyruvate oxidase, as well as acetyl-CoA synthetase, the enzyme that converts extracellular acetate to acetyl-CoA. The hypoxia-inducible transcription factorArcA, acting independently of acetate metabolism, is also required for maximum lifespan extension in the lipA and lpdA mutants, indicating that these mutations promote entry into a mode normally associated with a low-oxygen environment. Gonidakis et al. 2010 20707865 [DOI icon]doi:10.1111/j.1474-9726.2010.00618.x [Lifespan Observations Database icon]Lifespan Observations Database (ID: 3947)
4 OB_002654 Escherichia coli BW25113
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
lipAEscherichia colideletion / null-unknownunknown
increased 1100.00% 18 1.5 days median 37 E. coli strains lacking tricarboxylic acid (TCA)-cycle-related genes exhibit longer stationary-phase survival and increased resistance to heat stress compared to wild-type. Extended lifespan in the sdhA mutant, lacking subunit A of succinate dehydrogenase, is associated with the reduced production of superoxide and increased stress resistance. On the other hand, the longer lifespan of the lipoic acid synthase mutant (lipA) is associated with reduced oxygen consumption and requires the acetate-producing enzyme pyruvate oxidase, as well as acetyl-CoA synthetase, the enzyme that converts extracellular acetate to acetyl-CoA. The hypoxia-inducible transcription factorArcA, acting independently of acetate metabolism, is also required for maximum lifespan extension in the lipA and lpdA mutants, indicating that these mutations promote entry into a mode normally associated with a low-oxygen environment. Gonidakis et al. 2010 20707865 [DOI icon]doi:10.1111/j.1474-9726.2010.00618.x [Lifespan Observations Database icon]Lifespan Observations Database (ID: 3946)
5 OB_006131 Caenorhabditis elegans
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
age-1Caenorhabditis elegans174762Mutation-wildtypeyes
increased 900.00% maximum C. elegans strains bearing homozygous nonsense mutations in the age-1 gene, which encodes the class-I phosphatidylinositol 3-kinase catalytic subunit (PI3KCS), produce progeny that were thought to undergo obligatory developmental arrest. After prolonged developmental times at 15 – 20C, they mature into extremely long-lived adults with near-normal feeding rates and motility. They survive to a median of 145–190 days at 20C, with nearly 10-fold extension of both median and maximum adult lifespan relative to N2DRM, a long-lived wild-type stock into which the null mutant was outcrossed. PI3K-null adults, although a little less thermotolerant, are considerably more resistant to oxidative and electrophilic stresses than worms bearing normal or less long-lived alleles. Their unprecedented factorial gains in survival, under both normal and toxic environments, are attributed to elimination of residual and maternally-contributed PI3KCS or its products, and consequent modification of kinase signaling cascades. Ayyadevara et al. 2008 17996009 [DOI icon]doi:10.1111/j.1474-9726.2007.00348.x [GenAge icon]GenAge
6 OB_003401 Caenorhabditis elegans N2DRM
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
age-1Caenorhabditis elegans174762loss of functionmg44unknownunknown
increased 800.66% 136 15.1 days mean 20 Lifespan of age-1(mg44) outcrossed 6 generations was approximately 800% greater than control. Ayyadevara et al. 2008 17996009 [DOI icon]doi:10.1111/j.1474-9726.2007.00348.x [Lifespan Observations Database icon]Lifespan Observations Database (ID: 4828)
7 OB_004902 Podospora anserina Wa32
Other Ageing Factor NameAdditional Information
Dietary restrictionReducing sodium acetate from 2% to 0.2%.
increased 733.33% 175 21 days mean Dietary restriction by reducing availability of the carbon source increases life span in P. anserina. van Diepeningen et al. 2010 20026344 [DOI icon]doi:10.1016/j.mad.2009.12.002 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 4292)
8 OB_004901 Podospora anserina Wa32
Other Ageing Factor NameAdditional Information
Dietary restrictionReducing sodium acetate from 2% to 0.02%.
increased 714.29% 171 21 days mean Dietary restriction by reducing availability of the carbon source increases life span in P. anserina. van Diepeningen et al. 2010 20026344 [DOI icon]doi:10.1016/j.mad.2009.12.002 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 4291)
9 OB_004904 Podospora anserina Wa32
Other Ageing Factor NameAdditional Information
Dietary restrictionReducing fructose from 2% to 0.02%.
increased 691.30% 182 23 days mean Dietary restriction by reducing availability of the carbon source increases life span in P. anserina. van Diepeningen et al. 2010 20026344 [DOI icon]doi:10.1016/j.mad.2009.12.002 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 4294)
10 OB_004899 Podospora anserina s
Other Ageing Factor NameAdditional Information
Dietary restrictionReducing sodium acetate from 2% to 0.2%.
increased 672.22% 139 18 days mean Dietary restriction by reducing availability of the carbon source increases life span in P. anserina. van Diepeningen et al. 2010 20026344 [DOI icon]doi:10.1016/j.mad.2009.12.002 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 4289)
11 OB_004900 Podospora anserina s
Other Ageing Factor NameAdditional Information
Dietary restrictionReducing sodium acetate from 2% to 0.02%.
increased 638.89% 133 18 days mean Dietary restriction by reducing availability of the carbon source increases life span in P. anserina. van Diepeningen et al. 2010 20026344 [DOI icon]doi:10.1016/j.mad.2009.12.002 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 4290)
12 OB_004903 Podospora anserina Wa32
Other Ageing Factor NameAdditional Information
Dietary restrictionReducing fructose from 2% to 0.2%.
increased 565.22% 153 23 days mean Dietary restriction by reducing availability of the carbon source increases life span in P. anserina. van Diepeningen et al. 2010 20026344 [DOI icon]doi:10.1016/j.mad.2009.12.002 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 4293)
13 OB_002999 Caenorhabditis elegans N2
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
daf-2Caenorhabditis elegans175410unknowne1370unknownunknown
Other Ageing Factor NameAdditional Information
Axenic mediumCulture on non-bacterial, semi-dened, axenic food sources.
increased 531.25% 90.9 14.4 days mean 24 hermaphrodite Comparisons of longevity of wild type and mutants grown on bacteria vs. axenic culture. Houthoofd et al. 2003 12954481 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 4333)
14 OB_004331 Saccharomyces cerevisiae BY4743
Compound NameQuantityQuantity Unit
Methanol200millimolar
Other Ageing Factor NameAdditional Information
Transfer to watertransfer to water at day 2 of the experiment
increased 500.00% 24 4 days median 30 matalpha Addition of 200 mM methanol had no effect on life span extension from transfer to water at day 2 of the experiment, but addition of 200 mM ethanol reduced life span extension. Burtner et al. 2009 19305133 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 2119)
15 OB_004790 Saccharomyces cerevisiae BY4743
Other Ageing Factor NameAdditional Information
pH 6 buffering0.1 M citrate-phosphate buffer added at day 2 of the experiment
increased 500.00% 24 4 days median 30 matalpha Buffering the culture medium to pH 6 with 0.1 M citrate-phosphate buffer at day 2 of adulthood increases chronological life span. Burtner et al. 2009 19305133 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 2111)
16 OB_004794 Saccharomyces cerevisiae BY4743
Other Ageing Factor NameAdditional Information
Transfer to watertransfer of cells to water at day 2 of the experiment
increased 500.00% 24 4 days median 30 matalpha Addition of acetic acid as needed to maintain a concentration of 10 mM in solution prevented life span extension from transfer to water Burtner et al. 2009 19305133 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 2116)
17 OB_004795 Saccharomyces cerevisiae BY4743
Other Ageing Factor NameAdditional Information
Dietary restriction0.05% glucose
increased 500.00% 24 4 days median 30 matalpha Addition of acetic acid as needed to maintain a concentration of 10 mM in solution prevented life span extension from transfer to water or dietary restriction. Burtner et al. 2009 19305133 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 2117)
18 OB_004813 Saccharomyces cerevisiae BY4742
Other Ageing Factor NameAdditional Information
Dietary restrictionReduced glucose in the medium to 0.05%
increased 500.00% 36 6 days median 30 matalpha Reduced glucose (below 2%) increased chronological life span while increased glucose (more than 2%) shortened life span. Murakami et al. 2008 18314444 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 2158)
19 OB_004788 Saccharomyces cerevisiae W303AR5
Other Ageing Factor NameAdditional Information
pH 6 bufferingMedia was buffered to pH 6 in 0.1 M citrate-phosphate buffer
increased 450.00% 22 4 days median 30 matalpha Buffering the culture medium to pH 6 with citrate-phosphate or MES buffers increases the chronological life span of cells grown in synthetic defined medium. Burtner et al. 2009 19305133 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 2109)
20 OB_004897 Podospora anserina s
Other Ageing Factor NameAdditional Information
Dietary restrictionReducing fructose from 2% to 0.02%.
increased 450.00% 132 24 days mean Dietary restriction by reducing availability of the carbon source increases life span in P. anserina. van Diepeningen et al. 2010 20026344 [DOI icon]doi:10.1016/j.mad.2009.12.002 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 4287)
21 OB_004898 Podospora anserina s
Other Ageing Factor NameAdditional Information
Dietary restrictionReducing fructose from 2% to 0.2%.
increased 450.00% 132 24 days mean Dietary restriction by reducing availability of the carbon source increases life span in P. anserina. van Diepeningen et al. 2010 20026344 [DOI icon]doi:10.1016/j.mad.2009.12.002 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 4288)
22 OB_000793 Podospora anserina
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
cyc1Podospora anserinaloss of function-unknownunknown
increased 400.00% days mean Mutants live longer by more than 400%. Mutants have a reduced growth rate, a reduced reactive oxygen species production. Sellem et al. 2007 17081785 [DOI icon]doi:10.1016/j.fgb.2006.09.005 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 2050)
23 OB_004783 Saccharomyces cerevisiae W303AR5
Other Ageing Factor NameAdditional Information
Dietary restriction0.05% glucose
increased 400.00% 20 4 days median 30 mata Dietary restriction by reducing the glucose concentration of the medium from 2% to 0.05% glucose increases the chronological life span of cells grown in synthetic defined medium. Burtner et al. 2009 19305133 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 2104)
24 OB_004791 Saccharomyces cerevisiae BY4743
Other Ageing Factor NameAdditional Information
Transfer to waterTransfer of cells to water at day 2 of the experiment
increased 400.00% 25 5 days median 30 matalpha Transferring cells to water at day 2 of the experiment increases chronological life span. Burtner et al. 2009 19305133 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 2112)
25 OB_004792 Saccharomyces cerevisiae BY4743
Other Ageing Factor NameAdditional Information
Transfer to waterTransfer of cells to water at day 4 of the experiment
increased 400.00% 25 5 days median 30 matalpha Transferring cells to water at day 4 of the experiment increases chronological life span. Burtner et al. 2009 19305133 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 2113)
26 OB_004793 Saccharomyces cerevisiae BY4743
Other Ageing Factor NameAdditional Information
Dietary restriction0.05% glucose
increased 400.00% 25 5 days median 30 matalpha Dietary restriction by reducing the glucose concentration of the medium from 2% to 0.05% glucose increases the chronological life span of cells grown in synthetic defined medium. Burtner et al. 2009 19305133 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 2114)
27 OB_004334 Saccharomyces cerevisiae BY4743
Compound NameQuantityQuantity Unit
Ethanol200millimolar
Other Ageing Factor NameAdditional Information
pH 6 buffer0.1 mM citrate-phosphate buffer pH 6
Transfer to watertransfer to water at day 2 of the experiment
increased 380.00% 24 5 days median 30 matalpha Addition of 200 mM ethanol reduced life span extension from transfer to water, but this was suppressed by buffering the solution to pH 6. Burtner et al. 2009 19305133 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 2122)
28 OB_004782 Saccharomyces cerevisiae BY4743
Other Ageing Factor NameAdditional Information
Dietary restriction0.05% glucose
increased 380.00% 24 5 days median 30 diploid Dietary restriction by reducing the glucose concentration of the medium from 2% to 0.05% glucose increases the chronological life span of cells grown in synthetic defined medium. Burtner et al. 2009 19305133 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 2103)
29 OB_004796 Saccharomyces cerevisiae BY4743
Other Ageing Factor NameAdditional Information
pH 6 buffer0.1 mM citrate-phosphate buffer pH 6
Transfer to watertransfer to water at day 2 of the experiment
increased 380.00% 24 5 days median 30 matalpha Addition of 200 mM ethanol reduced life span extension from transfer to water, but this was suppressed by buffering the solution to pH 6. Burtner et al. 2009 19305133 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 2123)
30 OB_002657 Escherichia coli BW25113
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
atpCEscherichia colideletion / null-unknownunknown
increased 366.67% 7 1.5 days median 37 E. coli strains lacking tricarboxylic acid (TCA)-cycle-related genes exhibit longer stationary-phase survival and increased resistance to heat stress compared to wild-type. Extended lifespan in the sdhA mutant, lacking subunit A of succinate dehydrogenase, is associated with the reduced production of superoxide and increased stress resistance. On the other hand, the longer lifespan of the lipoic acid synthase mutant (lipA) is associated with reduced oxygen consumption and requires the acetate-producing enzyme pyruvate oxidase, as well as acetyl-CoA synthetase, the enzyme that converts extracellular acetate to acetyl-CoA. The hypoxia-inducible transcription factorArcA, acting independently of acetate metabolism, is also required for maximum lifespan extension in the lipA and lpdA mutants, indicating that these mutations promote entry into a mode normally associated with a low-oxygen environment. Gonidakis et al. 2010 20707865 [DOI icon]doi:10.1111/j.1474-9726.2010.00618.x [Lifespan Observations Database icon]Lifespan Observations Database (ID: 3949)
31 OB_000915 Caenorhabditis elegans N2
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
daf-12Caenorhabditis elegans181263unknownm20unknownunknown
daf-2Caenorhabditis elegans175410unknowne1391unknownunknown
increased 340.49% 71.8 16.3 days median 22.5 hermaphrodite 15 temperature-sensitive and 1 nonconditional daf-2 allele were characterized to investigate the extent of daf-2 mutant defects and to examine whether specific mutant traits correlate with each other. The greatest longevity seen in daf-2 mutant adults was approximately three times that of wild type. The temperature-sensitive daf-2 mutants fell into two overlapping classes, including eight class 1 mutants, which are Daf-c, Age, and Itt, and exhibit low levels of L1 arrest at 25.5 degrees. Seven class 2 mutants also exhibit the class 1 defects as well as some or all of the following: reduced adult motility, abnormal adult body and gonad morphology, high levels of embryonic and L1 arrest, production of progeny late in life, and reduced brood size. The strengths of the Daf-c, Age, and Itt phenotypes largely correlated with each other but not with the strength of class 2-specific defects. This suggests that the DAF-2 receptor is bifunctional. Gems et al. 1998 9725835 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 2222)
32 OB_004784 Saccharomyces cerevisiae PSY316AT
Other Ageing Factor NameAdditional Information
Dietary restriction0.05% glucose
increased 340.00% 22 5 days median 30 matalpha Dietary restriction by reducing the glucose concentration of the medium from 2% to 0.05% glucose increases the chronological life span of cells grown in synthetic defined medium. Burtner et al. 2009 19305133 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 2105)
33 OB_004785 Saccharomyces cerevisiae DBY746
Other Ageing Factor NameAdditional Information
Dietary restriction0.05% glucose
increased 340.00% 22 5 days median 30 mata Dietary restriction by reducing the glucose concentration of the medium from 2% to 0.05% glucose increases the chronological life span of cells grown in synthetic defined medium. Burtner et al. 2009 19305133 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 2106)
34 OB_004787 Saccharomyces cerevisiae PSY316AT
Other Ageing Factor NameAdditional Information
pH 6 bufferingMedia was buffered to pH 6 in 0.1 M citrate-phosphate buffer
increased 340.00% 22 5 days median 30 matalpha Buffering the culture medium to pH 6 with citrate-phosphate or MES buffers increases the chronological life span of cells grown in synthetic defined medium. Burtner et al. 2009 19305133 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 2108)
35 OB_000866 Saccharomyces cerevisiae BY4742
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
SIR2Saccharomyces cerevisiae851520deletion / null-unknownunknown
Other Ageing Factor NameAdditional Information
Dietary restrictionReduced glucose in the medium to 0.05%
increased 328.57% 30 7 days median 30 matalpha Deletion of sir2, hst1, hst2, hst3, hst4 or all 5 sirtuins at the same time had no effect on chronological life span and did not reduce life span extension from dietary restriction at 0.5% or 0.05% glucose. Murakami et al. 2008 18314444 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 2171)
36 OB_000878 Saccharomyces cerevisiae BY4742
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
hst4Saccharomyces cerevisiae851772deletion / null-unknownunknown
Other Ageing Factor NameAdditional Information
Dietary restrictionReduced glucose in the medium to 0.05%
increased 328.57% 30 7 days median 30 matalpha Deletion of sir2, hst1, hst2, hst3, hst4 or all 5 sirtuins at the same time had no effect on chronological life span and did not reduce life span extension from dietary restriction at 0.5% or 0.05% glucose. Murakami et al. 2008 18314444 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 2183)
37 OB_000879 Saccharomyces cerevisiae BY4742
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
hst3Saccharomyces cerevisiae854190deletion / null-unknownunknown
Other Ageing Factor NameAdditional Information
Dietary restrictionReduced glucose in the medium to 0.05%
increased 328.57% 30 7 days median 30 matalpha Deletion of sir2, hst1, hst2, hst3, hst4 or all 5 sirtuins at the same time had no effect on chronological life span and did not reduce life span extension from dietary restriction at 0.5% or 0.05% glucose. Murakami et al. 2008 18314444 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 2184)
38 OB_004818 Saccharomyces cerevisiae BY4742
Other Ageing Factor NameAdditional Information
Dietary restrictionReduced glucose in the medium to 0.05%
increased 328.57% 30 7 days median 30 matalpha Dietary restriction by reducing the glucose concentration of the medium from 2% to 0.5% or 0.05% increased chronological life span in the BY4742, PSY316AR, and W303AR5 strain backgrounds. Murakami et al. 2008 18314444 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 2163)
39 OB_003583 Caenorhabditis elegans N2
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
daf-2Caenorhabditis elegans175410loss of functione1370unknownunknown
increased 313.33% 62 15 days max 20 Maximum lifespan of daf-2(e1370) mutant was approximately 315% longer than control. Fierro-González et al. 2011 21334311 [DOI icon]doi:10.1016/j.bbrc.2011.02.079 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 5017)
40 OB_000784 Saccharomyces cerevisiae
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
MEC1Saccharomyces cerevisiae852433loss of function-unknownunknown
increased 300.00% si Mutants have a 3-fold reduced mean and maximum chronological lifespan under conditions of medium depletion. Weinberger et al. 2007 17710147 [DOI icon]doi:10.1371/journal.pone.0000748 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 2042)
41 OB_003038 Schizosaccharomyces pombe
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
Pka1Schizosaccharomyces pombeloss of function-unknownunknown
increased 300.00% Knockouts exhibited a three-fold increase in lifespan. Roux et al. 2006 16822282 [DOI icon]doi:10.1111/j.1474-9726.2006.00225.x [Lifespan Observations Database icon]Lifespan Observations Database (ID: 4383)
42 OB_003766 Drosophila melanogaster
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
GSRDrosophila melanogasterover-expression-unknownunknown
increased 300.00% Overexpression of glutathione reductase in transgenic flies results in increased lifespan by 300%. Mockett et al. 1999 10506576 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 532)
43 OB_003312 Caenorhabditis elegans N2
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
age-1Caenorhabditis elegans174762loss of functionm305unknownunknown
increased 297.48% 47.3 11.9 days mean 22.5 Mean lifespan of age-1(m305) mutant was 297% greater than wild type. Houthoofd et al. 2005 15771612 [DOI icon]doi:10.1111/j.1474-9726.2005.00150.x [Lifespan Observations Database icon]Lifespan Observations Database (ID: 4736)
44 OB_001119 Caenorhabditis elegans N2
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
osm-5Caenorhabditis elegans180585unknownp813unknownunknown
Other Ageing Factor NameAdditional Information
Somatic gonad ablationZ1, Z4 laser ablation at L1 larval stage
increased 295.65% 72.8 18.4 days mean 20 hermaphrodite Several (but not all) mutations that cause defects in sensory cilia or their support cells, or in sensory signal transduction, extend lifespan. These mutations do not further extend long-lived daf-2 mutants Apfeld et al. 1999 10617200 [DOI icon]doi:10.1038/45544 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 2439)
45 OB_003076 Saccharomyces cerevisiae X2180-1A
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
X2180-1BSaccharomyces cerevisiaeunknown-unknownunknown
increased 286.67% 58 15 divisions max 30 mata Two strains with different lifespan characteristics (X2180-1A is shorter lived than X2180-1B) were crossed to form a diploid strain. The diploid strain had lifespan similar to X2180-1B, the longer lived of the two haploid parents. Jazwinski et al. 1989 2698814 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 4447)
46 OB_000872 Saccharomyces cerevisiae BY4742
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
HST1Saccharomyces cerevisiae854086deletion / null-unknownunknown
Other Ageing Factor NameAdditional Information
Dietary restrictionReduced glucose in the medium to 0.05%
increased 275.00% 30 8 days median 30 matalpha Deletion of sir2, hst1, hst2, hst3, hst4 or all 5 sirtuins at the same time had no effect on chronological life span and did not reduce life span extension from dietary restriction at 0.5% or 0.05% glucose. Murakami et al. 2008 18314444 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 2177)
47 OB_000873 Saccharomyces cerevisiae BY4742
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
HST2Saccharomyces cerevisiae856092deletion / null-unknownunknown
Other Ageing Factor NameAdditional Information
Dietary restrictionReduced glucose in the medium to 0.05%
increased 275.00% 30 8 days median 30 matalpha Deletion of sir2, hst1, hst2, hst3, hst4 or all 5 sirtuins at the same time had no effect on chronological life span and did not reduce life span extension from dietary restriction at 0.5% or 0.05% glucose. Murakami et al. 2008 18314444 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 2179)
48 OB_004823 Saccharomyces cerevisiae BY4742
Other Ageing Factor NameAdditional Information
Dietary restrictionReduced glucose in the medium to 0.05%
increased 275.00% 30 8 days median 30 matalpha Deletion of sir2, hst1, hst2, hst3, hst4 or all 5 sirtuins at the same time had no effect on chronological life span and did not reduce life span extension from dietary restriction at 0.5% or 0.05% glucose. Murakami et al. 2008 18314444 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 2178)
49 OB_001118 Caenorhabditis elegans N2
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
osm-5Caenorhabditis elegans180585unknownp813unknownunknown
Other Ageing Factor NameAdditional Information
Germ cell ablationZ2, Z3 laser ablation at L1 larval stage
increased 272.83% 68.6 18.4 days mean 20 hermaphrodite Several (but not all) mutations that cause defects in sensory cilia or their support cells, or in sensory signal transduction, extend lifespan. These mutations do not further extend long-lived daf-2 mutants Apfeld et al. 1999 10617200 [DOI icon]doi:10.1038/45544 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 2438)
50 OB_002545 Caenorhabditis elegans N2
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
daf-2Caenorhabditis elegans175410loss of functione1370unknownunknown
pept-1Caenorhabditis elegans180919deletion / nulllg601unknownunknown
increased 266.67% 77 21 days max 25 hermaphrodite Double mutation extended maximum lifespan 266%. Meissner et al. 2004 15155758 [DOI icon]doi:10.1074/jbc.M403415200 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 3798)
51 OB_002147 Caenorhabditis elegans N2
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
daf-2Caenorhabditis elegans175410loss of function(e1370)IIIunknownunknown
nuo-4Caenorhabditis elegans176001RNAi knockdown-unknownunknown
increased 265.10% 70.1 19.2 days mean 20 hermaphrodite For calculating relative effect of lifespan in mutant background, an average of all N2 data in Supplemental Table I was used since no experiment matched control was provided. RNA knockdown increased daf-2 lifespan by 82% relative to daf-2 lifespan alone. Hansen et al. 2005 16103914 [DOI icon]doi:10.1371/journal.pgen.0010017 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 3432)
52 OB_000881 Saccharomyces cerevisiae BY4742
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
HST1Saccharomyces cerevisiae854086deletion / null-unknownunknown
HST2Saccharomyces cerevisiae856092deletion / null-unknownunknown
hst3Saccharomyces cerevisiae854190deletion / null-unknownunknown
hst4Saccharomyces cerevisiae851772deletion / null-unknownunknown
SIR2Saccharomyces cerevisiae851520deletion / null-unknownunknown
Other Ageing Factor NameAdditional Information
Dietary restrictionReduced glucose in the medium to 0.05%
increased 257.14% 25 7 days median 30 matalpha Deletion of sir2, hst1, hst2, hst3, hst4 or all 5 sirtuins at the same time had no effect on chronological life span and did not reduce life span extension from dietary restriction at 0.5% or 0.05% glucose. Murakami et al. 2008 18314444 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 2187)
53 OB_004824 Saccharomyces cerevisiae BY4742
Other Ageing Factor NameAdditional Information
Dietary restrictionReduced glucose in the medium to 0.05%
increased 257.14% 25 7 days median 30 matalpha Deletion of sir2, hst1, hst2, hst3, hst4 or all 5 sirtuins at the same time had no effect on chronological life span and did not reduce life span extension from dietary restriction at 0.5% or 0.05% glucose. Murakami et al. 2008 18314444 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 2186)
54 OB_002291 Caenorhabditis elegans N2
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
daf-2Caenorhabditis elegans175410loss of functione1370unknownunknown
isp-1Caenorhabditis elegans177609loss of functionqm150unknownunknown
increased 248.39% 108 31 days max 20 hermaphrodite Double mutation leads to increased (250%) maximum lifespan. Feng et al. 2001 11709184 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 3566)
55 OB_002529 Caenorhabditis elegans N2
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
daf-2Caenorhabditis elegans175410loss of functione1370unknownunknown
pept-1Caenorhabditis elegans180919deletion / nulllg601unknownunknown
increased 248.18% 38.3 11 days mean 25 hermaphrodite Double mutation increased lifespan 250%. Meissner et al. 2004 15155758 [DOI icon]doi:10.1074/jbc.M403415200 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 3783)
56 OB_000777 Mus musculus
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
Chek2Mus musculus50883loss of function-unknownunknown
increased 240.00% mean female Double mutants for chk2 extend lifespan of female mice hyopmorphic for Brca1 by at least 240%. Mice hypomorphic for Brca1 and double mutant for chk2 exhibit signs of premature ageing. Cao et al. 2006 16675955 [DOI icon]doi:10.1038/sj.emboj.7601115 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 2036)
57 OB_002146 Caenorhabditis elegans N2
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
daf-2Caenorhabditis elegans175410loss of function(e1370)IIIunknownunknown
nuo-3Caenorhabditis elegans178404RNAi knockdown-unknownunknown
increased 239.58% 65.2 19.2 days mean 20 hermaphrodite For calculating relative effect of lifespan in mutant background, an average of all N2 data in Supplemental Table I was used since no experiment matched control was provided. RNA knockdown increased daf-2 lifespan by 69% relative to daf-2 lifespan alone. Hansen et al. 2005 16103914 [DOI icon]doi:10.1371/journal.pgen.0010017 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 3431)
58 OB_002294 Caenorhabditis elegans N2
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
daf-2Caenorhabditis elegans175410loss of functione1370unknownunknown
isp-1Caenorhabditis elegans177609loss of functionqm150unknownunknown
increased 238.09% 71 21 days max 25 hermaphrodite Double mutation leads to increased (230%) maximum lifespan. Feng et al. 2001 11709184 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 3569)
59 OB_000794 Caenorhabditis elegans daf-2(e1370);daf-12(m20)
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
daf-12Caenorhabditis elegans181263loss of function-unknownunknown
daf-2Caenorhabditis elegans175410loss of function-unknownunknown
increased 236.59% 41.4 12.3 days mean 25.5 Mutations in both daf-2 and daf-12 nearly quadruple lifespan. Larsen et al. 1995 7789761 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 2051)
60 OB_004376 Saccharomyces cerevisiae BY4741
Compound NameQuantityQuantity Unit
Spermidine4mM
increased 233.33% 50 15 days median 30 mata Addition of spermidine at day 1 of the experiment increases chronological life span. Eisenberg et al. 2009 19801973 [DOI icon]doi:10.1038/ncb1975 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 2545)
61 OB_002293 Caenorhabditis elegans N2
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
daf-2Caenorhabditis elegans175410loss of functione1370unknownunknown
isp-1Caenorhabditis elegans177609loss of functionqm150unknownunknown
increased 231.45% 41.1 12.4 days mean 25 hermaphrodite Double mutation leads to increased (230%) mean lifespan. Feng et al. 2001 11709184 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 3568)
62 OB_000824 Caenorhabditis elegans N2
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
clk-1Caenorhabditis elegans175729loss of functionqm30unknownunknown
daf-2Caenorhabditis elegans175410loss of functione1370unknownunknown
Other Ageing Factor NameAdditional Information
Q-less dietFeeding worms GD1, an E. coli strain unable to synthesize coenzyme Q beginnign at L4.
increased 225.00% 52 16 days median 25 hermaphrodite Feeding C. elegans the E. coli strain GD1, which is unable to synthesize coenzyme Q, increases life span relative to animals fed the standard E. coli strain OP50.Life span extension from GD1 also occurred in the following mutant backgrounds: daf-12(m20), daf-16(m26), clk-1(e2519), clk-1(qm30), clk-1(qm51), daf-2(e1370); clk-1(qm30) and daf-2(e1370); daf-12(m20)It should be noted that GD1 is a different strain background than OP50 Larsen et al. 2002 11778046 [DOI icon]doi:10.1126/science.1064653 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 2085)
63 OB_002245 Caenorhabditis elegans N2
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
daf-2Caenorhabditis elegans175410loss of function(e1370)IIIunknownunknown
nuo-2Caenorhabditis elegans172363RNAi knockdown-unknownunknown
increased 219.08% 48.5 15.2 days mean 25 hermaphrodite For calculating relative effect of lifespan in mutant background, an average of all N2 data in Supplemental Table I was used since no experiment matched control was provided. RNA knockdown increased lifespan of daf-2 mutants by 93% relative to daf-2 mutants alone. Hansen et al. 2005 16103914 [DOI icon]doi:10.1371/journal.pgen.0010017 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 3520)
64 OB_002248 Caenorhabditis elegans N2
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
cco-1Caenorhabditis elegans172832RNAi knockdown-unknownunknown
daf-2Caenorhabditis elegans175410loss of function(e1370)IIIunknownunknown
increased 217.76% 48.3 15.2 days mean 25 hermaphrodite For calculating relative effect of lifespan in mutant background, an average of all N2 data in Supplemental Table I was used since no experiment matched control was provided. RNA knockdown increased lifespan of daf-2 mutants by 92% relative to daf-2 mutants alone. Hansen et al. 2005 16103914 [DOI icon]doi:10.1371/journal.pgen.0010017 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 3523)
65 OB_002151 Caenorhabditis elegans N2
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
atp-5Caenorhabditis elegans179541RNAi knockdown-unknownunknown
daf-2Caenorhabditis elegans175410loss of function(e1370)IIIunknownunknown
increased 208.85% 59.3 19.2 days mean 20 hermaphrodite For calculating relative effect of lifespan in mutant background, an average of all N2 data in Supplemental Table I was used since no experiment matched control was provided. RNA knockdown increased daf-2 lifespan by 29% relative to daf-2 lifespan alone. Hansen et al. 2005 16103914 [DOI icon]doi:10.1371/journal.pgen.0010017 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 3436)
66 OB_002137 Caenorhabditis elegans N2
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
daf-2Caenorhabditis elegans175410loss of function(e1370)IIIunknownunknown
sams-1Caenorhabditis elegans181370RNAi knockdown-unknownunknown
increased 208.33% 59.2 19.2 days mean 20 hermaphrodite For calculating relative effect of lifespan in mutant background, an average of all N2 data in Supplemental Table I was used since no experiment matched control was provided. RNA knockdown increased daf-2 lifespan by 55% relative to daf-2 lifespan alone. Hansen et al. 2005 16103914 [DOI icon]doi:10.1371/journal.pgen.0010017 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 3423)
67 OB_003077 Saccharomyces cerevisiae X2180-1A
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
X2180-1A x X2180-1BSaccharomyces cerevisiaeunknown-unknownunknown
increased 206.67% 46 15 divisions max 30 mata Two strains with different lifespan characteristics (X2180-1A is shorter lived than X2180-1B) were crossed to form a diploid strain. The diploid strain had lifespan similar to X2180-1B, the longer lived of the two haploid parents. Jazwinski et al. 1989 2698814 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 4448)
68 OB_002152 Caenorhabditis elegans N2
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
asb-2Caenorhabditis elegans180718RNAi knockdown-unknownunknown
daf-2Caenorhabditis elegans175410loss of function(e1370)IIIunknownunknown
increased 205.21% 58.6 19.2 days mean 20 hermaphrodite For calculating relative effect of lifespan in mutant background, an average of all N2 data in Supplemental Table I was used since no experiment matched control was provided. RNA knockdown increased daf-2 lifespan by 52% relative to daf-2 lifespan alone. Hansen et al. 2005 16103914 [DOI icon]doi:10.1371/journal.pgen.0010017 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 3437)
69 OB_002148 Caenorhabditis elegans N2
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
daf-2Caenorhabditis elegans175410loss of function(e1370)IIIunknownunknown
nuo-5Caenorhabditis elegans178735RNAi knockdown-unknownunknown
increased 203.12% 58.2 19.2 days mean 20 hermaphrodite For calculating relative effect of lifespan in mutant background, an average of all N2 data in Supplemental Table I was used since no experiment matched control was provided. RNA knockdown increased daf-2 lifespan by 26% relative to daf-2 lifespan alone. Hansen et al. 2005 16103914 [DOI icon]doi:10.1371/journal.pgen.0010017 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 3433)
70 OB_002143 Caenorhabditis elegans N2
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
daf-2Caenorhabditis elegans175410loss of function(e1370)IIIunknownunknown
ril-2Caenorhabditis elegans179725RNAi knockdown-unknownunknown
increased 202.08% 58 19.2 days mean 20 hermaphrodite For calculating relative effect of lifespan in mutant background, an average of all N2 data in Supplemental Table I was used since no experiment matched control was provided. RNA knockdown increased daf-2 lifespan by 36% relative to daf-2 lifespan alone. Hansen et al. 2005 16103914 [DOI icon]doi:10.1371/journal.pgen.0010017 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 3429)
71 OB_000608 Saccharomyces cerevisiae DBY746
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
SCH9Saccharomyces cerevisiae856612deletion / null-unknownunknown
increased 200.00% 27 9 days median 30 Deletion of SCH9 or inactivating mutations (K441A or D556R) increased chronological life span approximately 3-fold. Fabrizio et al. 2001 11292860 [DOI icon]doi:10.1126/science.1059497 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 1876)
72 OB_000609 Saccharomyces cerevisiae DBY746
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
SCH9Saccharomyces cerevisiae856612loss of functionK441Aunknownunknown
increased 200.00% days median 30 Deletion of SCH9 or inactivating mutations (K441A or D556R) increased chronological life span approximately 3-fold. Fabrizio et al. 2001 11292860 [DOI icon]doi:10.1126/science.1059497 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 1877)
73 OB_000610 Saccharomyces cerevisiae DBY746
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
SCH9Saccharomyces cerevisiae856612loss of functionD556Runknownunknown
increased 200.00% days median 30 Deletion of SCH9 or inactivating mutations (K441A or D556R) increased chronological life span approximately 3-fold. Fabrizio et al. 2001 11292860 [DOI icon]doi:10.1126/science.1059497 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 1878)
74 OB_000785 Saccharomyces cerevisiae
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
RAD9Saccharomyces cerevisiae851803deletion / null-unknownunknown
increased 200.00% si mean Deletion results in mutants with a 2-fold decrease in mean and maximum chronological lifespan under conditions of nutrient depletion. Weinberger et al. 2007 17710147 [DOI icon]doi:10.1371/journal.pone.0000748 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 2043)
75 OB_004789 Saccharomyces cerevisiae BY4743
Other Ageing Factor NameAdditional Information
pH 6 bufferingMedia was buffered to pH 6 in 0.1 M citrate-phosphate buffer
increased 200.00% 15 5 days median 30 matalpha Buffering the culture medium to pH 6 with citrate-phosphate or MES buffers increases the chronological life span of cells grown in synthetic defined medium. Burtner et al. 2009 19305133 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 2110)
76 OB_005876 Saccharomyces cerevisiae
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
SCH9Saccharomyces cerevisiae856612Mutation-wildtypeyes
increased 200.00% unknown More than 3 fold extension of chronological lifespan, by day 9 of medium depletion all the WT yeast were dead while 50% sch9 mutants survived Weinberger et al. 2007 17710147 [DOI icon]doi:10.1371/journal.pone.0000748 [GenAge icon]GenAge
77 OB_007413 Caenorhabditis elegans
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
daf-2Caenorhabditis elegans175410Mutations-wildtypeyes
increased 200.00% unknown Mutants show a 2-3 fold increase in lifespan. Wolkow et al. 2000 11021802 [GenAge icon]GenAge
78 OB_001116 Caenorhabditis elegans N2
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
daf-10Caenorhabditis elegans184883unknownm79unknownunknown
Other Ageing Factor NameAdditional Information
Somatic gonad ablationZ1, Z4 laser ablation at L1 larval stage
increased 196.74% 54.6 18.4 days mean 20 hermaphrodite Several (but not all) mutations that cause defects in sensory cilia or their support cells, or in sensory signal transduction, extend lifespan. These mutations do not further extend long-lived daf-2 mutants Apfeld et al. 1999 10617200 [DOI icon]doi:10.1038/45544 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 2436)
79 OB_001115 Caenorhabditis elegans N2
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
daf-10Caenorhabditis elegans184883unknownm79unknownunknown
Other Ageing Factor NameAdditional Information
Germ cell ablationZ2, Z3 laser ablation at L1 larval stage
increased 192.94% 53.9 18.4 days mean 20 hermaphrodite Several (but not all) mutations that cause defects in sensory cilia or their support cells, or in sensory signal transduction, extend lifespan. These mutations do not further extend long-lived daf-2 mutants Apfeld et al. 1999 10617200 [DOI icon]doi:10.1038/45544 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 2435)
80 OB_003582 Caenorhabditis elegans N2
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
daf-2Caenorhabditis elegans175410loss of functione1370unknownunknown
increased 192.31% 38 13 days median 20 Median lifespan of daf-2(e1370) mutant was approximately 190% longer than control. Fierro-González et al. 2011 21334311 [DOI icon]doi:10.1016/j.bbrc.2011.02.079 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 5016)
81 OB_002350 Caenorhabditis elegans N2
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
daf-2Caenorhabditis elegans175410loss of functione1370unknownunknown
kri-1Caenorhabditis elegans191275RNAi knockdown-unknownunknown
rrf-3Caenorhabditis elegans174315loss of functionpk1426unknownunknown
increased 188.46% 52.5 18.2 days mean 20 hermaphrodite RNA interference increased lifespan 112% relative to double mutant lifespan. Berman et al. 2006 16530050 [DOI icon]doi:10.1016/j.cell.2006.01.039 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 3621)
82 OB_003447 Caenorhabditis elegans N2
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
daf-2Caenorhabditis elegans175410loss of functione1370unknownunknown
sea-2Caenorhabditis elegans173738loss of functionbp283unknownunknown
increased 188.00% 72 25 days max 20 Maximum lifespan of sea-2;daf-2 double mutant was approximately 188% longer than control. Huang et al. 2011 21471153 [DOI icon]doi:10.1242/dev.057109 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 4873)
83 OB_000825 Caenorhabditis elegans N2
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
clk-1Caenorhabditis elegans175729loss of functionqm30unknownunknown
daf-2Caenorhabditis elegans175410loss of functione1370unknownunknown
increased 187.50% 46 16 days median 25 hermaphrodite Feeding C. elegans the E. coli strain GD1, which is unable to synthesize coenzyme Q, increases life span relative to animals fed the standard E. coli strain OP50.Life span extension from GD1 also occurred in the following mutant backgrounds: daf-12(m20), daf-16(m26), clk-1(e2519), clk-1(qm30), clk-1(qm51), daf-2(e1370); clk-1(qm30) and daf-2(e1370); daf-12(m20)It should be noted that GD1 is a different strain background than OP50 Larsen et al. 2002 11778046 [DOI icon]doi:10.1126/science.1064653 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 2086)
84 OB_004337 Saccharomyces cerevisiae BY4742
Compound NameQuantityQuantity Unit
Sorbitol0-
increased 187.50% 23 8 days median 30 matalpha Osmotic stress in the form of 18% sorbitol or 0.3 M NaCl increased chronological life span. Murakami et al. 2008 18314444 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 2168)
85 OB_003313 Caenorhabditis elegans N2
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
aap-1Caenorhabditis elegans172141loss of functionm889unknownunknown
increased 187.40% 34.2 11.9 days mean 22.5 Mean lifespan of aap-1(m889) mutant was 187% greater than wild type. Houthoofd et al. 2005 15771612 [DOI icon]doi:10.1111/j.1474-9726.2005.00150.x [Lifespan Observations Database icon]Lifespan Observations Database (ID: 4737)
86 OB_003487 Caenorhabditis elegans N2
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
age-1Caenorhabditis elegans174762loss of functionhx546unknownunknown
Compound NameQuantityQuantity Unit
ThT (4-(3,6-dimethyl-1,3-benzothiazol-3-ium-2-yl)-N,N-dimethylan50M
increased 185.71% 30 10.5 days median 20 hermaphrodite Exposure to ThT (50M) lengthened median lifespan of age-1(hx546)II mutant approximately 186% relative to N2 control, approximately 88% relative to age-1(hx546)II lifespan. Alavez et al. 2011 21451522 [DOI icon]doi:10.1038/nature09873 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 4914)
87 OB_004814 Saccharomyces cerevisiae BY4742
Other Ageing Factor NameAdditional Information
Increased amino acids10X
increased 185.71% 20 7 days median 30 matalpha Increased amino acid content (10X) of the synthetic defined medium increased chronological life span while reduced amino acid content had little effect. Murakami et al. 2008 18314444 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 2159)
88 OB_007464 Caenorhabditis elegans
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
daf-4Caenorhabditis elegans175781Mutation-wildtypeyes
increased 185.00% maximum Mutation in adults increased mean lifespan by up to 120% and maximum lifespan by up to 185%. Shaw et al. 2007 17900898 [DOI icon]doi:10.1016/j.cub.2007.08.058 [GenAge icon]GenAge
89 OB_002142 Caenorhabditis elegans N2
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
daf-2Caenorhabditis elegans175410loss of function(e1370)IIIunknownunknown
ril-1Caenorhabditis elegans179958RNAi knockdown-unknownunknown
increased 184.90% 54.7 19.2 days mean 20 hermaphrodite For calculating relative effect of lifespan in mutant background, an average of all N2 data in Supplemental Table I was used since no experiment matched control was provided. RNA knockdown increased daf-2 lifespan by 42% relative to daf-2 lifespan alone. Hansen et al. 2005 16103914 [DOI icon]doi:10.1371/journal.pgen.0010017 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 3428)
90 OB_003574 Caenorhabditis elegans N2
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
daf-2Caenorhabditis elegans175410loss of functione1370unknownunknown
increased 184.13% 35.8 12.6 days mean 20 Mean lifespan of daf-2(e1370) mutant was approximately 185% longer than control. Fierro-González et al. 2011 21334311 [DOI icon]doi:10.1016/j.bbrc.2011.02.079 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 5009)
91 OB_002538 Caenorhabditis elegans N2
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
daf-2Caenorhabditis elegans175410loss of functione1370unknownunknown
pept-1Caenorhabditis elegans180919deletion / nulllg601unknownunknown
increased 184.00% 71 25 days max 20 hermaphrodite Double mutation extended maximum lifespan 184%. Meissner et al. 2004 15155758 [DOI icon]doi:10.1074/jbc.M403415200 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 3791)
92 OB_000755 Caenorhabditis elegans lrs-2(mg312)
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
lrs-2Caenorhabditis elegansloss of function-unknownunknown
increased 178.57% 39 14 days mean A mutation that impaired mitochondrial function was associated with approximately 180% longer lifespan. Lee et al. 2003 12447374 [DOI icon]doi:10.1038/ng1056 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 2015)
93 OB_002343 Caenorhabditis elegans N2
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
daf-2Caenorhabditis elegans175410loss of functione1370unknownunknown
kri-1Caenorhabditis elegans191275RNAi knockdown-unknownunknown
increased 178.17% 54.8 19.7 days mean 20 hermaphrodite RNA interference increased mutant lifespan by 123% relative to mutant lifespan. Berman et al. 2006 16530050 [DOI icon]doi:10.1016/j.cell.2006.01.039 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 3615)
94 OB_002149 Caenorhabditis elegans N2
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
cco-2Caenorhabditis elegans176707RNAi knockdown-unknownunknown
daf-2Caenorhabditis elegans175410loss of function(e1370)IIIunknownunknown
increased 176.56% 53.1 19.2 days mean 20 hermaphrodite For calculating relative effect of lifespan in mutant background, an average of all N2 data in Supplemental Table I was used since no experiment matched control was provided. RNA knockdown increased daf-2 lifespan by 38% relative to daf-2 lifespan alone. Hansen et al. 2005 16103914 [DOI icon]doi:10.1371/journal.pgen.0010017 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 3434)
95 OB_000950 Caenorhabditis elegans N2
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
daf-2Caenorhabditis elegans175410loss of functionrh50;e1370unknownunknown
increased 176.47% 70.5 25.5 days max 22.5 hermaphrodite Mutation increased maximum lifespan 176%. Gerisch et al. 2001 11740945 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 2256)
96 OB_001217 Caenorhabditis elegans N2
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
daf-2Caenorhabditis elegans175410loss of function-unknownunknown
odr-3Caenorhabditis elegans179806loss of function-unknownunknown
increased 175.56% 49.6 18 days mean hermaphrodite Loss of function mutations increased lifespan 175%. Lans et al. 2007 17187771 [DOI icon]doi:10.1016/j.ydbio.2006.11.028 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 2540)
97 OB_003421 Caenorhabditis elegans N2
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
cdc-25.1Caenorhabditis elegans172353RNAi knockdown-unknownunknown
daf-2Caenorhabditis elegans175410loss of functione1370unknownunknown
Other Ageing Factor NameAdditional Information
Pathogen resistanceWorms were infected at 25 C by feeding on PA14 lawns.
increased 175.32% 362.6 131.7 mean 25 Lifespan of daf-2(e1370) mutant was approximately 175% longer than N2 control on cdc-25.1 RNAi background. Evans et al. 2008 18782349 [DOI icon]doi:10.1111/j.1474-9726.2008.00435.x [Lifespan Observations Database icon]Lifespan Observations Database (ID: 4846)
98 OB_002420 Caenorhabditis elegans N2
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
daf-2Caenorhabditis elegans175410RNAi knockdown-unknownunknown
lin-14Caenorhabditis elegans181337gain of functionn179tsunknownunknown
increased 175.00% 33 12 days max 25 hermaphrodite RNA knockdown increased maximum lifespan relative to both wild type and mutant lifespan. Boehm et al. 2005 16373574 [DOI icon]doi:10.1126/science.1115596 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 3685)
99 OB_004338 Saccharomyces cerevisiae BY4742
Compound NameQuantityQuantity Unit
Sodium chloride300millimolar
increased 175.00% 22 8 days median 30 matalpha Osmotic stress in the form of 18% sorbitol or 0.3 M NaCl increased chronological life span. Murakami et al. 2008 18314444 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 2169)
100 OB_002246 Caenorhabditis elegans N2
Gene SymbolSpeciesNCBI Gene IDAllele TypeAllele NameReference Allele TypeAllele Change
cyc-1Caenorhabditis elegans172582RNAi knockdown-unknownunknown
daf-2Caenorhabditis elegans175410loss of function(e1370)IIIunknownunknown
increased 173.03% 41.5 15.2 days mean 25 hermaphrodite For calculating relative effect of lifespan in mutant background, an average of all N2 data in Supplemental Table I was used since no experiment matched control was provided. RNA knockdown increased lifespan of daf-2 mutants by 65% relative to daf-2 mutants alone. Hansen et al. 2005 16103914 [DOI icon]doi:10.1371/journal.pgen.0010017 [Lifespan Observations Database icon]Lifespan Observations Database (ID: 3521)